SAB5900202

Alpaca Anti-PD-L1, recombinant single domain Antibody

Recombinant Alpaca(VHH) Nano antibody

• Synonym(s): B7-H, B7H1, PD-L1, PDCD1LG1, PDL1
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: alpaca
• Quality Level: 100
• recombinant: expressed in E. coli
• conjugate: unconjugated
• antibody form: affinity purified VHH antibody
• clone: PL03, monoclonal
• Assay: >95% (SDS-PAGE)
• form: lyophilized solid
• mol wt: 14.4 kDa; ~ 15-18 kDa (SDS PAGE under reducing condition)
• purified by: immobilized metal affinity chromatography (IMAC)
• species reactivity: human, (Cynomolgus macaques)
• concentration: 1 mg/mL
• technique(s): ELISA: 1:10000-1:100000; flow cytometry: 1:50-1:100; immunofluorescence: 1:200-1:800; immunohistochemistry: 1:200-1:800; western blot: 1:1000-1:3000
• color: colorless
• pH: ( in PBS, pH 7.4)
• isotype: VHH
• Protein ID accession no.: Q9NZQ7.1
• UniProt accession no.: Q9NZQ7
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... CD274(29126)

Description

General description

PD-L1, also known as CD274 or B7H1, stands for programmed cell death ligand 1. It is a type I transmembrane protein that is thought to repress immune responses by binding to its receptor (PD1), thus inhibiting T-cell activation, proliferation, and cytokine production. It contains V-like and C-like immunoglobulin domains. PD-L1 expression is regulated by various cytokines, such as TNF-α or LPS (ISSN: 1848-7718). Increased expression of this protein in certain types of cancers, e.g., renal cell carcinoma or colon cancer, correlates with poor prognosis.Alpaca derived anti-PD-L1 VHH single domain antibody (molecular weight: 14.4kDa), expressed in E. coli under conditions free from animal derived components. It has a 6His tag at its C-terminal.
Single-domain antibody (sdAb), also called nano-antibody, is the variable region isolated from the antigen-binding variable domain (VHH) of camelid heavy-chain-only antibody. It lacks light chains and a first constant domain(CH1). This configuration confers a number of unique advantages over conventional monoclonal antibodies. The small size of just 15 kDa (2~4 nm), single-domain antibody is the smallest antigen-binding fragment that can easily penetrate tissues, allowing them to bind epitopes that are inacessible to conventional antibodies. The small size and monovalency makes it a superior tool in microsopy. These single-domain antibodies offer superior chemical (wide variety of pH, urea, etc) and thermal stability and increased solubility than conventional antibodies. All our antibodies are tested to ensure the highest level of specificity, sensitivity and reproducibility.

Specificity

Human Programmed Death-Ligand 1 (PD-L1)

Immunogen

Recombinant human PD-L1 (Phe19-Tyr134) protein

Application

ELISA, WB, FC, IHC, IF

Packaging

50 µg per vial / 100 µg per vial

Physical form

Affinity purified VHH single domain antibody lyophilized from PBS, pH7.5

Reconstitution

Reconstitute the lyophilized antibody with sterile deionized water or with 50% glycerol (ACS grade or better) to a stock solution of 1 mg/mL. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing.

Storage and Stability

Stable for 1 year at 4°C from date of receipt.

Other Notes

Storage at 4°C; Do not freeze.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable