The FLAG epitope, is an eight amino acid protein with an enterokinase-cleavage site.
Specificity
The antibody detects transfected proteins contanining 6×His tag.
Immunogen
Synthetic peptide: 18XHis conjugated to KLH
Application
Further analysis of the protein can be achieved using an antibody specific to the 6 X histidine in techniques such as western blotting.The antibody recognizes the His-tag fused to the carboxy- termini of targeted proteins in transfected or transformed cells.
The Anti-6-His antibody has been used in western blotting.
Biochem/physiol Actions
The antibody recognizes the His-tag fused to the carboxy- termini of targeted proteins in transfected or transformed cells.
Features and Benefits
Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.
Physical form
Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Bacteria respond dynamically to the changes in zinc availability. Repression by the Bacillus subtilis transcription factor Zur requires Zn(II), which binds with negative cooperativity to two regulatory sites per dimer to form, sequentially, Zur2:Zn3 and Zur2:Zn4 forms of the repressor.
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