S4760

N-Succinyl-Ala-Ala-Ala-p-nitroanilide

elastase substrate, chromogenic, ≥98% (HPLC), powder

• Synonym(s): N-Succinyl-L-alanyl-L-alanyl-L-alanine 4-nitroanilide, N-Succinyl-tri-L-alanine 4-nitroanilide
• Empirical Formula (Hill Notation): C₁₉H₂₅N₅O₈
• CAS Number: 52299-14-6
• Molecular Weight: 451.43
• Beilstein: 2928351
• EC Number: 257-823-5
• MDL number: MFCD00036774
• UNSPSC Code: 12352204
• PubChem Substance ID: 24899617
• NACRES: NA.32

Properties

• Product Name: N-Succinyl-Ala-Ala-Ala-p-nitroanilide, elastase substrate
• Quality Level: 200
• Assay: ≥98% (HPLC)
• form: powder
• solubility: DMF: 25 mg/mL, clear, colorless to light yellow
• storage temp.: 2-8°C
• SMILES string: C[C@H](NC(=O)CCC(O)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](C)C(=O)Nc1ccc(cc1)[N+]([O-])=O
• InChI: 1S/C19H25N5O8/c1-10(20-15(25)8-9-16(26)27)17(28)21-11(2)18(29)22-12(3)19(30)23-13-4-6-14(7-5-13)24(31)32/h4-7,10-12H,8-9H2,1-3H3,(H,20,25)(H,21,28)(H,22,29)(H,23,30)(H,26,27)/t10-,11-,12-/m0/s1
• InChI key: GVUGADOWXGKRAE-SRVKXCTJSA-N

Description

General description

N-Succinyl-Ala-Ala-Ala-p-nitroanilide is a chromogenic substrate for chymotrypsin-like serine protease,[1] pancreatic elastase[2] and serine endopeptidase.[3]

Application

N-Succinyl-Ala-Ala-Ala-p-nitroanilide has been used as a substrate

• for screening elastase activity in lyophilized earthworm fibrinolytic enzymes[4]
• for quantifying elastase activity in sheep milk[5]
• in screening honeybee inhibitors in serine protease inhibition assay[6]

Substrates

Elastase substrate.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves