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Q1126

Sigma-Aldrich

Q Sepharose Fast Flow

preswollen, 45-165 μm (wet), exclusion limit ~4,000,000 Da

Synonym(s):

Q Sepharose

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About This Item

MDL number:
UNSPSC Code:
47101511
NACRES:
NA.56

form

preswollen

Quality Level

technique(s)

RNA extraction: suitable

particle size

45-165 μm (wet)

pore size

~4,000,000 Da exclusion limit

capacity

0.18-0.24 mmol Cl-/mL, packed gel

storage temp.

2-8°C

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General description

−Ο−CH2CHOHCH2OCH2CHOHCH2N+(CH3)3

Application

Q Sepharose is used in protein chromatography, ion exchange chromatography and anion exchange media. Q Sepharose has been used to study compounds of plant defense with applications for natural control of phytopathogenic fungi. Q Sepharosehas also been used to develop an efficient method for extracting high-quality mRNA from soil and to study the immunomodulatory proteins from garlic (Allium sativum).

Physical form

Preswollen in 20% ethanol

Legal Information

Sepharose is a trademark of Cytiva

Pictograms

Flame

Signal Word

Warning

Hazard Statements

Hazard Classifications

Flam. Liq. 3

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Flash Point(F)

100.4 - 109.4 °F - closed cup

Flash Point(C)

38 - 43 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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E G Campos et al.
International journal for parasitology, 29(5), 655-662 (1999-07-15)
Cytochrome c peroxidase oxidises hydrogen peroxide using cytochrome c as the electron donor. This enzyme is found in yeast and bacteria and has been also described in the trematodes Fasciola hepatica and Schistosoma mansoni. Using partially purified cytochrome c peroxidase
Shikimate dehydrogenase from pepper (<I>Capsicum annuum</I>) seedlings. Purification and properties.
Diaz, J., and Merino, F.
Physiologia Plantarum, 100(1), 147-152 (1997)
Fatima Clement et al.
International immunopharmacology, 10(3), 316-324 (2009-12-17)
Garlic (Allium sativum), an important medicinal spice, displays a plethora of biological effects including immunomodulation. Although some immunomodulatory proteins from garlic have been described, their identities are still unknown. The present study was envisaged to isolate immunomodulatory proteins from raw
Carsten Mettel et al.
Applied and environmental microbiology, 76(17), 5995-6000 (2010-07-14)
Here, we report an efficient method for extracting high-quality mRNA from soil. Key steps in the isolation of total RNA were low-pH extraction (pH 5.0) and Q-Sepharose chromatography. The removal efficiency of humic acids was 94 to 98% for all
Irini Bournazou et al.
The Journal of clinical investigation, 119(1), 20-32 (2008-11-27)
Apoptosis is a noninflammatory, programmed form of cell death. One mechanism underlying the non-phlogistic nature of the apoptosis program is the swift phagocytosis of the dying cells. How apoptotic cells attract mononuclear phagocytes and not granulocytes, the professional phagocytes that

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