Propidium iodide is soluble in water at a concentration of 1 mg/mL.
P4170
Propidium iodide
≥94.0% (HPLC)
Synonym(s):
3,8-Diamino-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridinium diiodide
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10 MG
₹4,600.63
25 MG
₹9,179.60
100 MG
₹22,656.73
250 MG
₹48,322.80
500 MG
₹63,618.53
1 G
₹1,58,618.73
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10 MG
₹4,600.63
25 MG
₹9,179.60
100 MG
₹22,656.73
250 MG
₹48,322.80
500 MG
₹63,618.53
1 G
₹1,58,618.73
About This Item
Empirical Formula (Hill Notation):
C27H34I2N4
CAS Number:
Molecular Weight:
668.39
Beilstein:
3843838
EC Number:
MDL number:
UNSPSC Code:
41106305
PubChem Substance ID:
NACRES:
NA.77
Recommended Products
Quality Level
Assay
≥94.0% (HPLC)
form
powder
mp
220-225 °C (dec.) (lit.)
storage temp.
2-8°C
SMILES string
[I-].[I-].CC[N+](C)(CC)CCC[n+]1c(-c2ccccc2)c3cc(N)ccc3c4ccc(N)cc14
InChI
1S/C27H33N4.2HI/c1-4-31(3,5-2)17-9-16-30-26-19-22(29)13-15-24(26)23-14-12-21(28)18-25(23)27(30)20-10-7-6-8-11-20;;/h6-8,10-15,18-19,29H,4-5,9,16-17,28H2,1-3H3;2*1H/q+1;;/p-1
InChI key
XJMOSONTPMZWPB-UHFFFAOYSA-M
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General description
Application
Features and Benefits
This compound is a featured product for Apoptosis research. Click here to discover more featured Apoptosis products. Learn more about bioactive small molecules for other areas of research at sigma.com/discover-bsm.
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Muta. 2
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Methods in molecular biology (Clifton, N.J.), 2279, 213-223 (2021-03-09)
Annexin V and propidium iodide staining is widely used for determining the cellular death through apoptosis. In the presence of Ca2+ ions, annexin V has a strong binding affinity for phosphatidylserine, a membrane phospholipid that during apoptosis is translocated from
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It has been reported that calpain/caspase-mediated apoptosis induced by endoplasmic reticulum stress (ERS) in hepatic stellate cells (HSCs) by previous studies. At present, the activation of HSC is an important cause of liver fibrosis, and the induction of HSC apoptosis
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Precise glycosylation plays a crucial and distinctive role in thymic T cell development. The core fucosylation is dramatically up-regulated at the transition from CD4-CD8- (DN) to CD4+CD8+ (DP) in the thymic development. Ablation of core fucosylation in T cells did
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Forensic science international. Genetics, 46, 102269-102269 (2020-03-11)
Forensic DNA typing from touched or handled items in routine casework is increasing as the sensitivity of detection techniques improves. Our understanding of the cellular/acellular content of touch deposits and the origins of the DNA therein is still limited. This
Articles
Cell cycle phases (G1, S, G2, M) regulate cell growth, DNA replication, and division in proliferating cells.
Apoptosis regulation involves multiple pathways and molecules for cellular homeostasis.
Related Content
Nancy-520 for DNA Detection and Quantitation
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What is the procedure for dissolving this product?
1 answer-
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Is it necessary to carry out an RNase treatment before staining with propidium iodide in my immunofluorescence assay on insect tissue fixed in 4% paraformaldehyde? Additionally, is there the complete staining protocol for propidium iodide in immunofluorescence using a confocal microscope?
1 answer-
The product has not been validated for immunofluorescence assay in our laboratory to establish a reference protocol. In cases where propidium iodide binds to both DNA and RNA, the use of RNase is common to differentiate DNA staining from undesired RNA staining. For an application protocol, one may refer to a citation of this product in an immunofluorescence assay that utilizes RNase to visualize DNA-specific staining. The citation is as follows: J Cancer. 2020 Mar 5;11(11):3274-3287. The article discusses the dual role of BI 2536, a small-molecule inhibitor targeting PLK1, in inducing apoptosis and attenuating autophagy in neuroblastoma cells. The PMID for the article is 32231733.
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Is this reagent suited for viability assays at FACS?
1 answer-
While this product is not tested for any specific application, propidium iodide is commonly used in cell-based assays such as FACS. Please see the link below to review publications detailing the use of this item in flow cytometry:
https://scholar.google.com/scholar?hl=en&as_sdt=0%2C22&q=%22p4170%22+flow+cytometry&btnG=Helpful?
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What is the method to dissolve this product?
1 answer-
Propidium iodide can be dissolved in water at a concentration of 1 mg/mL. Please see the sample Certificate of Analysis for solubility information: https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/P4170-BULKWXBF1544V.pdf
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