This product is intended for use as a component for the Total Dietary Fiber Assay kit, TDF-100A. Unfortunately, the activity is not determined. See the link below to review Protease from Bacillus licheniformis products that are tested for activity:
https://www.sigmaaldrich.com/substance/proteasefrombacilluslicheniformis123459014011
P3910
Protease from Bacillus licheniformis
lyophilized powder, for use in Total Dietary Fiber Assay, TDF-100A
Synonym(s):
Proteinase from Bacillus licheniformis, Subtilisin A
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500 MG
₹63,147.90
5 X 500 MG
₹2,27,316.90
₹63,147.90
Available to ship on11 April 2025Details
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500 MG
₹63,147.90
5 X 500 MG
₹2,27,316.90
About This Item
₹63,147.90
Available to ship on11 April 2025Details
form
lyophilized powder
quality
for use in Total Dietary Fiber Assay, TDF-100A
mol wt
27 kDa
application(s)
food and beverages
shipped in
wet ice
storage temp.
2-8°C
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Specificity
Subtilisin A is a member of the Serine S8 Endoproteinase family. It has broad specificity with a preference for a large uncharged residue in the P1 position. It hydrolyzes native and denatured proteins, and is active under alkaline conditions.
Application
This is a proteolytic enzyme isolated from the fermentation of Bacillus licheniformis. It is a serine endoproteinase with a broad specificity towards native and denatured proteins, and is active under alkaline conditions. It is for use in Total Dietary Fiber Assays (TDF-100A).
Biochem/physiol Actions
Protease catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate .
Physical properties
Subtilisin is a non-glycosylated single polypeptide chain without disulfide bonds and has a molecular weight of 27 KDa.
Unit Definition
One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per minute at pH 7.5 at 37°C.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Target Organs
Respiratory system
Storage Class Code
11 - Combustible Solids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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