N2787

Monoclonal Anti-Neurofilament 160 antibody produced in mouse

~2 mg/mL, clone RMO44, purified immunoglobulin, buffered aqueous solution

• Synonym(s): Anti-Neurofilament Medium Chain
• MDL number: MFCD01322393
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: RMO44, monoclonal
• form: buffered aqueous solution
• species reactivity: human, mouse, rat, zebrafish
• concentration: ~2 mg/mL
• technique(s): immunohistochemistry: suitable; microarray: suitable; western blot: 1-2 μg/mL using extract of SHSy5y human neuroblastoma cells
• isotype: IgG1
• UniProt accession no.: P07197
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... NEFM(4741) ; mouse ... Nefm(18040) ; rat ... Nefm(24588)

Description

General description

Monoclonal Anti- Neurofilament 160 (mouse IgG1 isotype) is derived from the hybridoma RMO44 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with purified mid-sized rat neurofilament (NF-M) subunit. Neurofilaments are one of the five major groups of intermediate filaments (Ifs) and are found predominantly in cells or tissues of neuronal origin. They are composed of three major proteins of apparent molecular weights 68 kDa, 160 kDa and 200 kDa.

Neurofilament 160 (NEFM) is one among the most abundant filaments in post-mitotic neurons.

Immunogen

purified mid-sized rat neurofilament (NF-M) subunit.

Application

Monoclonal Anti-Neurofilament 160 antibody produced in mouse has been used in:

• enzyme-linked immunosorbent assay (ELISA)
• western blot analysis
• immunohistochemistry
• antibody labelling

Biochem/physiol Actions

Neurofilament 160 (NEFM) associates with the dopamine receptor.

Neurofilament proteins are synthesized in the neuronal perikarya, assembled to form filaments and then slowly transported within the axons towards the synaptic terminals. These molecules undergo post-translational modifications, which results in their heterogeneity including different levels of phosphorylation. The phosphorylation of neurofilament polypeptides has been suggested to modulate their function by influencing their interaction with cytoplasmic organelles.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable