MAK192
Cholesterol Efflux Assay Kit
Sufficient for 100 Fluorometric tests
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About This Item
UNSPSC Code:
12161503
Recommended Products
application(s)
cosmetics
food and beverages
pharmaceutical
detection method
fluorometric
relevant disease(s)
cardiovascular diseases
storage temp.
−20°C
General description
Reverse Cholesterol Transport (RCT) is the mechanism by which the peripheral lipid burden is reduced by transport to liver and its fecal excretion. The first important step in RCT is the cholesterol efflux from macrophage-derived foam cells present in atherosclerotic plaques. The cholesterol efflux is critical for the maintenance of net cholesterol balance in arterial walls and in the reduction of proinflammatory responses triggered by lipid-laden macrophages.
Features and Benefits
Compatible with high-throughput handling systems.
Suitability
Suitable for the determination of cholesterol efflux in cells.
Principle
The Cholesterol Efflux Assay Kit is a high-throughput screening assay that measures cholesterol efflux in cells using fluorescent-labeled cholesterol (λEx = 482 nm/ λEm = 515 nm). It is suitable for serum, isolated, or recombinant lipoprotein samples. It may also be used to screen small molecules for their effect on cholesterol efflux and therefore, may be a valuable tool in drug discovery programs.
Kit Components Only
Product No.
Description
- Labeling Reagent
- Equilibration Buffer
- Reagent A
- Reagent B
- Cell Lysis Buffer
- Positive Control
- Serum Treatment Reagent
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2
Storage Class Code
10 - Combustible liquids
Flash Point(F)
188.6 °F - closed cup
Flash Point(C)
87 °C - closed cup
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Journal of cellular and molecular medicine, 23(8), 5259-5269 (2019-05-24)
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