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MAK030

Sigma-Aldrich

Phosphate Colorimetric Kit

sufficient for 500 colorimetric tests

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 500 colorimetric tests

application(s)

cosmetics
environmental
food and beverages

detection method

colorimetric

storage temp.

room temp

General description

Phosphate is an essential component in living organisms and contributes to a variety of biological functions, including structural roles within nucleic acids, cellular membranes, and bone. Phosphate is also important in the transport of cellular energy, nucleic acid metabolism, and signal transduction. Hyperphosphatemia, a condition of excess phosphate levels in the blood, can lead to calcification of organs and interference with usage of other inorganic ions, such as iron, calcium, magnesium, and zinc.

The Phosphate Colorimetric Assay Kit provides a simple and direct procedure for measuring phosphate in a variety of samples. Phosphate reacts with a chromogenic complex, which results in a colorimetric (650 nm) product proportional to the amount of phosphate present. Phosphate concentrations between 1 μM and 1 mM, with a lower limit of detection of 0.1 nmol, can be directly determined.

Application

Phosphate colorimetric kit has been used to measure the phosphate concentration in a variety of samples.[1][2][3]

Suitability

Suitable for measuring phosphate levels in a variety of samples including serum and urine

Principle

The Phosphate Colorimetric Assay Kit provides a simple and direct procedure for measuring phosphate in a variety of samples. Phosphate reacts with a chromogenic complex, which results in a colorimetric (650 nm) product proportional to the amount of phosphate present. Phosphate concentrations between 1 μM and 1 mM, with a lower limit of detection of 0.1 nmol, can be directly determined.

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Pictograms

Corrosion

Signal Word

Warning

Hazard Statements

Hazard Classifications

Aquatic Chronic 3 - Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2

Storage Class Code

8A - Combustible corrosive hazardous materials


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Questions

1–3 of 3 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. SDSの有害性情報は各成分のSDSを参照とあるが、成分情報(項目3)も表示なく参照できません。成分情報を教えていただきたく。

    1 answer
    1. This kit includes only two components - the phosphate standard and the phosphate reagent. Both components are listed in the SDS. See the link below to review the Safety Data Sheet:
      https://www.sigmaaldrich.com/sds/sigma/mak030

      See the link below to reivew the technical bulletin which includes the list of components:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/406/002/mak030bul.pdf

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