I3019
Interleukin-10 from mouse
>97% (SDS-PAGE), recombinant, expressed in E. coli, suitable for cell culture
Synonym(s):
IL-10
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About This Item
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biological source
mouse
Quality Level
recombinant
expressed in E. coli
Assay
>97% (SDS-PAGE)
form
lyophilized powder
potency
0.01-1.50 ng/mL EC50
mol wt
~18 kDa
packaging
pkg of 5 μg
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
Gene Information
mouse ... Il10(16153)
Application
Interleukin-10 from mouse has been used as a standard in ELISA (enzyme-linked immunosorbent assay) cytokine assay.
Biochem/physiol Actions
Interleukin-10 is an important regulator of the functions of lymphoid and myeloid cells. IL-10 can block the activation of cytokine synthesis and several accessory functions of macrophages. Human and mouse IL-10 share a 73% sequence homology. However, human IL-10 acts on both human and mouse target cells, while mouse IL-10 has species-specific activity. In mouse, the cellular sources of IL-10 consist of Th2 cells, activated fetal thymocytes, macrophages, keratinocytes, LY-1+ (CD5+), and normal B cells. In human, the cellular sources of IL-10 consist of CD4+ T cells and T cell clones, thymocytes, B cells, B cell lymphomas, macrophages, mast cell lines and keratinocytes. IL-10 stimulates the growth of stem cells, mast cells and thymocytes. IL-10 enhances cytotoxic T cell development, and co-stimulates B cell differentiation and Ig secretion. IL-10 regulates angiogenesis by inducing the cell-type dependent expression of either angiogenic or angiostatic factors.
Interleukin-10 regulates lymphoid and myeloid cell functions. It blocks the activation of cytokine synthesis and several accessory functions of macrophages. IL-10 enhances cytotoxic T cell development and co-stimulates B cell differentiation and Ig secretion. IL-10 regulates angiogenesis by inducing cell-type dependent expression of either angiogenic or angiostatic factors.
Physical form
Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4 with 50 μg BSA per 1 μg as a carrier protein
Analysis Note
The biological activity of IL-10 is determined in a cell proliferation assay using MC/9 cells.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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Interleukins, released from leukocytes, include IL-1β playing crucial roles in inflammation, immune responses, and anti-tumor immunity.
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