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I1643

Sigma-Aldrich

Pyrophosphatase, Inorganic from baker′s yeast (S. cerevisiae)

powder, ≥500 units/mg protein (E1%/280)

Synonym(s):

Inorganic Pyrophosphatase, Pyrophosphate phosphohydrolase

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About This Item

CAS Number:
9024-82-2
Enzyme Commission number:
3.6.1.1 (BRENDA, IUBMB)
EC Number:
232-784-7
MDL number:
MFCD00131379
UNSPSC Code:
12352204
NACRES:
NA.54

form

powder

Quality Level

300

specific activity

≥500 units/mg protein (E1%/280)

mol wt

71 kDa

storage temp.

−20°C

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General description

Molecular Weight: 71 kDa (homodimer consisting of two equal subunits of molecular weight 32-35 kDa)
Extinction Coefficient: E1% = 14.5 (280 nm)
pI: 4.75
This ubiquitous enzyme serves to drive metabolic reactions that produce pyrophosphate, since these reactions typically have equilibrium constants near unity. The catalytic mechanism has been described in the literature. Inorganic pyrophosphatase is a metalloprotease that requires Mg2+ for maximal activity. Although the hydrolysis of inorganic pyrophosphate is specific in the presence of Mg2+, both ADP and ATP can be hydrolyzed if zinc is present.

Application

Inorganic pyrophosphatase (PPase) is a ubiquitous enzyme catalyzing the reaction PPi + H2O → 2Pi.
It plays an important role in protein, RNA, and DNA synthesis.
Pyrophosphatase has been used:
  • as a component of the reaction buffer used in aminoacylation assay to quantify incorporation of [3H]L-methionine into tRNA.
  • as a component of PCR mixture.
Pyrophosphatase has been used:
as a component of the reaction buffer used in aminoacylation assay to quantify incorporation of [3H]L-methionine into tRNA.
as a component of PCR mixture

Unit Definition

One unit will liberate 1.0 μmole of inorganic orthophosphate per min at pH 7.2 at 25 °C.

Physical form

Lyophilized powder containing 80-90% buffer salts

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Sigma-Aldrich

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Virginia P Ronchi et al.
The Journal of biological chemistry, 292(44), 18006-18023 (2017-09-20)
To understand the mechanism for assembly of Lys
Laura Pedró-Rosa et al.
Journal of biomolecular screening, 20(1), 122-130 (2014-08-29)
Improved therapies for the treatment of Trypanosoma brucei, the etiological agent of the neglected tropical disease human African trypanosomiasis, are urgently needed. We targeted T. brucei methionyl-tRNA synthetase (MetRS), an aminoacyl-tRNA synthase (aaRS), which is considered an important drug target
Enzymatic chemiluminescence assay for inorganic pyrophosphate.
Vuokko Jansson et al.
Analytical biochemistry, 304(1), 135-137 (2002-04-24)
Jin He et al.
Annals of neurology, 93(2), 244-256 (2022-09-12)
Despite the increasing number of genes associated with Charcot-Marie-Tooth (CMT) disease, many patients currently still lack appropriate genetic diagnosis for this disease. Autosomal dominant mutations in aminoacyl-tRNA synthetases (ARSs) have been implicated in CMT. Here, we describe causal missense mutations
Musleh M Muthana et al.
Chemical communications (Cambridge, England), 48(21), 2728-2730 (2012-02-07)
A promiscuous UDP-sugar pyrophosphorylase (BLUSP) was cloned from Bifidobacterium longum strain ATCC55813 and used efficiently with a Pasteurella multocida inorganic pyrophosphatase (PmPpA) with or without a monosaccharide 1-kinase for one-pot multienzyme synthesis of UDP-galactose, UDP-glucose, UDP-mannose, and their derivatives. Further
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