GE28-9893-35

HiLoad^® 16/600 Superdex^® 200 pg

Cytiva 28-9893-35, column L × I.D. 60 cm × 16 mm, 34 μm avg. part. size

• Synonym(s): 16/600 Superdex 200, HiLoad Superdex 200
• UNSPSC Code: 41115700
• NACRES: SB.52

Properties

• material: bed support (polyamide); glass column; glass fiber (polypropylene reinforced)
• mol wt: 10000-600000 Da (globular proteins)
• packaging: pkg of 1 ea
• manufacturer/tradename: Cytiva 28-9893-35
• parameter: <1.0 mL/min flow rate; <5 mL sample volume; 0.3 Mpa (42 psi)
• bed volume: 120 mL
• column L × I.D.: 60 cm × 16 mm
• matrix: agarose dextran composite; dextran-agarose particle platform; polymeric
• matrix active group: dextran-agarose phase
• avg. part. size: 34 μm
• cleaning: 1-14
• working range: 3-12
• separation technique: size exclusion (SEC)

Description

General description

HiLoad^® 16/600 Superdex^® 200 prep grade are prepacked XK columns designed for high-resolution preparative gel filtration chromatography. The column is an excellent choice for separating MAbs from dimers and contaminants.

Superdex^® prep grade (pg) is produced by covalent bonding of dextran to highly cross-linked agarose.The separation properties of these media are determined by the dextran component. Steep selectivity curves give excellent resolving power for peptides and proteins in the molecular weight ranges, Mr < 10 000 (Superdex^® 30 pg), Mr 3 000 to 70 000 (Superdex^® 75 pg), and Mr 10 000 600 000 (Superdex^® 200 pg). The medium combines high mechanical strength with high hydrophilicity, allowing high flow rates, and minimal nonspecific interactions.

Features and Benefits

• High resolution with short run times and good recovery.
• High capacity at high flow rates.
• Separations can be scaled up to production levels.
• Prepacked for convenience and reproducibility.
• 1/16” fittings for convenient connections to AKTA design systems.

Suitability

All commonly used buffers, 1 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 70 % ethanol, 30 % iso-propanol, 30 % acetonitrile

for high-resolution preparative gel filtration chromatography, e.g, separate mAbs from dimers and contaminants

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

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Legal Information

HiLoad is a registered trademark of Cytiva

Superdex is a registered trademark of Cytiva

Safety Information

• Pictograms: GHS02
• Signal Word: Warning
• Hazard Statements: H226
• Precautionary Statements: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Storage Class Code: 3 - Flammable liquids