G5885

Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides

lyophilized powder, >= 550 units/mg protein (biuret)

• Synonym(s): G-6-P-DH
• CAS Number: 9001-40-5
• Enzyme Commission number: 1.1.1.49 (BRENDA, IUBMB)
• EC Number: 232-602-6
• MDL number: MFCD00081656
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• biological source: bacterial (Leuconostoc mesenteroides)
• Quality Level: 300
• type: Type XXIV
• form: lyophilized powder
• specific activity: >= 550 units/mg protein (biuret)
• mol wt: 128 kDa
• composition: Protein, 15-40% biuret
• application(s): agriculture
• foreign activity: 6-Phosphogluconic dehydrogenase, hexokinase, NADH oxidase and NADPH oxidase ≤0.005%; PGI ≤0.01%
• storage temp.: 2-8°C

Description

General description

Glucose-6-Phophate Dehydrogenase (G-6-PDH) comprises His-Asp catalytic dyad and exists as a homodimer. Structurally, G-6-PDH encompasses a Rossmann dinucleotide binding fold in the coenzyme binding domain region. It also harbors a large β + α domain and has a unique aspartate residue at position 374.

Application

Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides has been used along with hexokinase in the determination of glucose from mice liver samples.

Biochem/physiol Actions

Glucose-6-Phophate Dehydrogenase (G-6-PDH) can utilize either nicotinamide adenine dinucleotide phosphate (NADP⁺) or NAD⁺ as coenzyme making it crucial for bacterial metabolism.

Glucose-6-phosphate dehydrogenase (G6PD) catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway.

Unit Definition

One unit will oxidize 1.0 μmole of D-glucose 6-phosphate to 6-phospho-D-gluconate per min in the presence of NAD at pH 7.8 at 30 °C.

Physical form

Lyophilized powder containing Ficoll and Tris buffer salts

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves