G4166
Glucose Isomerase from Streptomyces murinus
≥350 U/g
Synonym(s):
Sweetzyme® IT Extra, D-xylose ketol-isomerase
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About This Item
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
Recommended Products
biological source
Streptomyces sp. (S. murinus)
form
powder
specific activity
≥350 U/g
storage temp.
2-8°C
General description
Immobilized glucose isomerase produced from a selected strain of Streptomyces murinus.
Application
Glucose Isomerase from Streptomyces murinus has been used to isomerize xylose to xylulose during the production of dihydrogen from Xylose. It has also been used in the synthetic enzymatic pathway for dihydrogen production from sucrose, to catalyze isomerization of glucose to fructose.
Immobilized glucose isomerase produced from Streptomyces murinus was used for the isomerization of xylose. Glucose isomerase is used in the food industry to produce high-fructose corn syrup.
Biochem/physiol Actions
Few anaerobic bacteria, fungi and plants express an intracellular metalloenzyme called D-xylose isomerase (XI). Most bacteria use the enzyme D-xylose isomerase to transform D-xylose to D-xylulose. D-Xylose isomerase (XI) converts the aldo-sugars xylose and glucose to their keto analogs xylulose and fructose.
Glucose isomerase has wide variety of industrial applications such as producing high-fructose corn syrup (HFCS) and production of ethanol from hemicelluloses. In addition, it also facilitates the study of structure-function relationships by advanced biochemical and genetic engineering techniques.
Physical properties
0.33 g yields an approximate bed volume of 1ml
Unit Definition
one unit converts glucose to fructose at an initial rate of 1 μmole per min at standard analytical conditions
Legal Information
A product of Novozyme Corp.
Sweetzyme is a registered trademark of Novozymes Corp.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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Andrey Kovalevsky et al.
Acta crystallographica. Section D, Biological crystallography, 68(Pt 9), 1201-1206 (2012-09-06)
D-Xylose isomerase (XI) converts the aldo-sugars xylose and glucose to their keto analogs xylulose and fructose, but is strongly inhibited by the polyols xylitol and sorbitol, especially at acidic pH. In order to understand the atomic details of polyol binding
A D Kline et al.
Journal of molecular biology, 189(2), 377-382 (1986-05-20)
This is a preliminary report on the determination of the solution conformation of the alpha-amylase inhibitor Tendamistat by nuclear magnetic resonance and distance geometry calculations. A characterization is given of the complete polypeptide backbone fold and the side-chains of the
High-yield production of dihydrogen from xylose by using a synthetic enzyme cascade in a cell-free system
Martin DC, et al.
Angewandte Chemie (International Edition in English), 52(17), 4587-4590 (2013)
Philipp M Grande et al.
ChemSusChem, 5(7), 1203-1206 (2012-05-25)
Do you sea water? Water consumption will be a challenge in biorefineries, and the use of non-drinkable sources of water will be preferred. Herein, glucose is converted into 5-hydroxymethylfurfural (HMF) in a chemo-enzymatic one-pot, two-step procedure, involving immobilized glucose isomerase
Hang Zhou et al.
Metabolic engineering, 14(6), 611-622 (2012-08-28)
Xylose is the main pentose and second most abundant sugar in lignocellulosic feedstocks. To improve xylose utilization, necessary for the cost-effective bioconversion of lignocellulose, several metabolic engineering approaches have been employed in the yeast Saccharomyces cerevisiae. In this study, we
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