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F7425

Millipore

ANTI-FLAG® antibody produced in rabbit

ANTI-FLAG® antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-ddddk, Anti-dykddddk

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0.2 MG
₹63,036.90

₹63,036.90

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0.2 MG
₹63,036.90

About This Item

MDL number:
MFCD02262912
UNSPSC Code:
12352203
NACRES:
NA.32

₹63,036.90

In StockDetails

Request a Bulk Order

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

all

concentration

~0.8 mg/mL

technique(s)

dot blot: 1-2.5 μg/mL
immunoprecipitation (IP): 4-8 μg using amino terminal FLAG-BAP fusion protein from E. coli crude lysate
indirect immunofluorescence: 5-10 μg/mL using 293T cells transfected with a plasmid encoding FLAG-JNK
western blot (chemiluminescent): 1-2.5 μg/mL using an E. coli periplasmic extract expressing an N-terminal FLAG fusion protein

isotype

IgG

immunogen sequence

DYKDDDDK

shipped in

dry ice

storage temp.

−20°C

Related Categories

General description

The FLAG epitope, is an eight amino acid protein with an enterokinase-cleavage site.[1][2]
The rabbit Anti-FLAG polyclonal affinity antibody ANTI-FLAG recognizes the FLAG epitope located on FLAG fusion proteins. This antibody reacts with N-terminal, N-terminal-Met, and C-terminal FLAG fusion proteins.

Immunogen

FLAG; peptide sequence DYKDDDDK

Application

The antibody recognizes the FLAG epitope located on FLAG-tagged fusion proteins at the N-terminus or C-terminus, applying dot blot, immunoblotting[3][4], immunoprecipitation[3] and immunocytochemistry assays.
Learn more product details in our FLAG® application portal."

Biochem/physiol Actions

FLAG epitope plays a crucial role in immunoaffinity purification of fusion proteins.[1]

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Preparation Note

Purified by affinity chromatography on a column bearing the immunizing peptide.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Legal Information

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000395474
0000395474
0000236831
0000252651
0000252651

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Ryota Sato et al.
Frontiers in microbiology, 10, 1857-1857 (2019-08-29)
The envelope proteins of influenza A virus, hemagglutinin (HA) and neuraminidase (NA), play critical roles in viral entry to host cells and release from the cells, respectively. After protein synthesis, they are transported from the trans-Golgi network (TGN) to the
Makoto Miyazawa et al.
The Journal of biological chemistry, 286(22), 19191-19203 (2011-04-12)
The molecular chaperone prefoldin (PFD) is a complex comprised of six different subunits, PFD1-PFD6, and delivers newly synthesized unfolded proteins to cytosolic chaperonin TRiC/CCT to facilitate the folding of proteins. PFD subunits also have functions different from the function of
Martin S Taylor et al.
The Journal of biological chemistry, 288(45), 32211-32228 (2013-09-21)
Ghrelin O-acyltransferase (GOAT) is a polytopic integral membrane protein required for activation of ghrelin, a secreted metabolism-regulating peptide hormone. Although GOAT is a potential therapeutic target for the treatment of obesity and diabetes and plays a key role in other
Gouji Toyokawa et al.
Neoplasia (New York, N.Y.), 13(10), 887-898 (2011-10-27)
A number of histone methyltransferases have been identified and biochemically characterized, but the pathologic roles of their dysfunction in human diseases like cancer are not well understood. Here, we demonstrate that Wolf-Hirschhorn syndrome candidate 1 (WHSC1) plays important roles in
Dorothee A Vogt et al.
PLoS pathogens, 9(4), e1003302-e1003302 (2013-04-18)
The nonstructural protein NS5A has emerged as a new drug target in antiviral therapies for Hepatitis C Virus (HCV) infection. NS5A is critically involved in viral RNA replication that takes place at newly formed membranes within the endoplasmic reticulum (membranous
View All Related Papers

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Protein & Nucleic Acid Interactions

Protein and nucleic acid interaction reagents and resources for investing protein-RNA, protein-DNA, and protein-protein interactions and associated applications.

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Questions

1–9 of 9 Questions  

  1. Can this AB (F7425) used for mouse 30um thick section. mouse will be perfused with 4% PFA and than cryopreserved. thanks

    1 answer

    1. This product has been recommended for use in immunofluorescence and indirect immunofluorescence at 5-10 μg/mL using 293T cells transfected with a plasmid encoding FLAG-JNK. It has not been tested for use in IHC applications. The end-user will need to optimize the assay. Please see the link below to review the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/207/353/f7425dat-ms.pdf

      In general, antibodies work well on 5-15 uM sections, for 30 uM sections, blocking /permeabilization steps and antibody dilution, the incubation time will need to be optimized by end user. Please see the links below to review an alternative anti-Flag antibody that may be more suitable for this application. Note that this product is a mouse monoclonal antibody:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/175/747/f1804bul-ms.pdf
      https://www.sigmaaldrich.com/product/sigma/f1804

      Helpful?

  2. Is there a recommended isotype control for this antibody?

    1 answer

    1. Any rabbit IgG such as product I5006 would be an appropriate isotype control for this antibody.

      Helpful?

  3. What are the positive controls that can be used with Product F7425, ANTI-FLAG® from rabbit?

    1 answer

    1. The positive controls that can be used are as follows:Amino-terminal FLAG-BAP fusion protein, Product No. P7582Carboxy-terminal FLAG-BAP fusion protein, Product No. P7457Amino-terminal Met-FLAG-BAP fusion protein, Product No. P5975.

      Helpful?

  4. How to get rid of background when using Product F7465, ANTI-FLAG® from rabbit,?

    1 answer

    1. To reduce non-specific background on tissue staining, a small amount of Triton X-100 (Product No. X100 or T8787), can be added to the wash buffers and antibody incubation buffers. Try a Triton X-100 concentration of 0.1% to start. Tween 20 (Product No. P5927) can also be used.

      Helpful?

  5. What is the concentration of Product F7425, ANTI-FLAG® from rabbit?

    1 answer

    1. The concentration is 0.9 mg protein per ml.

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  6. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  7. Is Product F7425, ANTI-FLAG® from rabbit, a monoclonal or polyclonal?

    1 answer

    1. F7425 is a polyclonal antibody from rabbit.

      Helpful?

  8. Is there any references for the use of Product F7465, ANTI-FLAG® from rabbit, in immunohistochemistry or western blotting?

    1 answer

    1. K. Schafer and T. Braun, Monoclonal Anti-FLAG Antibodies React with a New Isoform of Rat Mg++ Dependent Protein Phosphatase. Biochemical and Biophysical Research Communications, 207, 708.

      Helpful?

  9. How much Product F7425, ANTI-FLAG® from rabbit, should be used in western blotting and ELISA applications?

    1 answer

    1. For a Western blot from a PAGE run (preferably) under denaturing conditions, a 1:400 dilution of this antibody should be used.  A 1-10 μg/ml concentration should be used when using thie antibody in an ELISA application.

      Helpful?

Reviews

Active Filters

  1. Nottingham
    • Review 1
    • Votes 0
    5 out of 5 stars.

    Western blotting

    I HAVE USED THIS ANTIBODY ALOT DURING MY RESEARCH. AND IT HAS WORKED PERFECTLY.

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