This product is assigned an expiration date of 4 years from the date of Quality Release. The expiration date is lot specific and reported on the product label and the Certificate of Analysis. The recommended storage temperature range is 2 - 8°C. Please see the link below to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/162/885/d9891pis.pdf
D9891
Doxycycline hyclate
Synonym(s):
6-Desoxy-5-hydroxytetracycline hydrochloride hemihydrate hemiethanolate, Doxycycline hydrochloride hemiethanolate hemihydrate
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1 G
₹6,094.48
5 G
₹18,056.10
10 G
₹23,501.08
25 G
₹30,959.50
100 G
₹1,20,157.50
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1 G
₹6,094.48
5 G
₹18,056.10
10 G
₹23,501.08
25 G
₹30,959.50
100 G
₹1,20,157.50
About This Item
Empirical Formula (Hill Notation):
C22H24N2O8 · HCl · 0.5H2O · 0.5C2H6O
CAS Number:
Molecular Weight:
512.94
Beilstein:
5702728
MDL number:
UNSPSC Code:
51284027
PubChem Substance ID:
NACRES:
NA.85
Recommended Products
biological source
synthetic
Quality Level
Assay
≥93.5% (HPLC)
95.0-102.0% anhydrous basis (ethanol free based)
form
powder
storage condition
(Tightly closed. Dry. )
color
yellow to yellow-green
antibiotic activity spectrum
Gram-negative bacteria
Gram-positive bacteria
mycoplasma
parasites
Mode of action
protein synthesis | interferes
storage temp.
2-8°C
SMILES string
Cl[H].Cl[H].[H]O[H].CCO.C[C@@H]1C2[C@H](O)C3[C@H](N(C)C)C(O)=C(C(N)=O)C(=O)[C@@]3(O)C(O)=C2C(=O)c4c(O)cccc14.C[C@@H]5C6[C@H](O)C7[C@H](N(C)C)C(O)=C(C(N)=O)C(=O)[C@@]7(O)C(O)=C6C(=O)c8c(O)cccc58
InChI
1S/2C22H24N2O8.C2H6O.2ClH.H2O/c2*1-7-8-5-4-6-9(25)11(8)16(26)12-10(7)17(27)14-15(24(2)3)18(28)13(21(23)31)20(30)22(14,32)19(12)29;1-2-3;;;/h2*4-7,10,14-15,17,25,27-29,32H,1-3H3,(H2,23,31);3H,2H2,1H3;2*1H;1H2/t2*7-,10?,14?,15-,17-,22-;;;;/m00..../s1
InChI key
HALQELOKLVRWRI-ZVACAFRPSA-N
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General description
Doxycycline Hyclate, a synthetic tetracycline antibiotic, demonstrates broad-spectrum antimicrobial activity against various bacteria by binding to the 30S and potentially the 50S ribosomal subunits. This binding disrupts the aminoacyl-tRNA binding to the mRNA-ribosome complex, effectively inhibiting bacterial protein synthesis, thereby halting growth and replication.
Beyond its antimicrobial effects, Doxycycline Hyclate serves as an inhibitor of matrix metalloproteinases (MMPs), enzymes crucial for collagen breakdown in connective tissues. Studies on corneal erosion and rheumatoid arthritis showcase its significant reduction in MMP-9 and MMP-8 levels, respectively. This MMP-inhibiting property helps preserve collagen integrity, preventing excessive tissue degradation and supporting tissue repair.
In cell biology, Doxycycline Hyclate impacts smooth muscle cells, enhancing adhesion and influencing the reorganization of fibrillar collagen matrices. Its influence on apicoplast gene expression in Plasmodium falciparum, the malaria-causing parasite, adds a dimension of significance in infectious disease research. Additionally, the inhibition of tissue formation in rat studies contributes to its relevance in understanding tissue development.
Moreover, Doxycycline Hyclate holds potential in metabolomics research, considering its multi-faceted effects on various cellular processes. In the broader context of biochemical research, its versatile actions make it a valuable tool for investigating molecular mechanisms and signaling pathways.
Beyond its antimicrobial effects, Doxycycline Hyclate serves as an inhibitor of matrix metalloproteinases (MMPs), enzymes crucial for collagen breakdown in connective tissues. Studies on corneal erosion and rheumatoid arthritis showcase its significant reduction in MMP-9 and MMP-8 levels, respectively. This MMP-inhibiting property helps preserve collagen integrity, preventing excessive tissue degradation and supporting tissue repair.
In cell biology, Doxycycline Hyclate impacts smooth muscle cells, enhancing adhesion and influencing the reorganization of fibrillar collagen matrices. Its influence on apicoplast gene expression in Plasmodium falciparum, the malaria-causing parasite, adds a dimension of significance in infectious disease research. Additionally, the inhibition of tissue formation in rat studies contributes to its relevance in understanding tissue development.
Moreover, Doxycycline Hyclate holds potential in metabolomics research, considering its multi-faceted effects on various cellular processes. In the broader context of biochemical research, its versatile actions make it a valuable tool for investigating molecular mechanisms and signaling pathways.
Application
Doxycycline hyclate has been used for the induction of glioma cells[1] and human embryonic kidney 293T cells (HEK293T).[2]
Doxycycline hyclate is a synthetic oxytetracycline derivative. It has been used to eliminate Borrelia burgdorferi and Anaplasma phagocytophilum in rodent reservoirs and to eliminate Ixodes scapularis ticks[3]. It is a broad spectrum inhibitor used to inhibit matrix metalloproteinases[4] (MMP), such as type 1 collagenase in studies on wound healing and tissue remodeling.
Biochem/physiol Actions
Mode of Action: Doxycycline hyclate inhibits the inflammatory response to the Lyme Disease Spirochete Borrelia burgdorferi.
Activity Spectrum: Effective against a broad spectrum inhibitor of matrix metalloproteinases in vivo.
Activity Spectrum: Effective against a broad spectrum inhibitor of matrix metalloproteinases in vivo.
Features and Benefits
- High-quality antibiotic suitable for multiple research applications
- Ideal for Cell Biology, Metabolomics, and Biochemical research.
Other Notes
For additional information on our range of Biochemicals, please complete this form.
also commonly purchased with this product
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT SE 3
Target Organs
Respiratory system
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Does this powder if stored in dark at 4 degree expires? Doxycycline hyclate, Product D9891
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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How can I determine the shelf life / expiration / retest date of this product?
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If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdfHelpful?
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What is the expiration date of Doxycycline hyclate(powder, not solution) from the date of purchase?
1 answer-
This product is not assigned an expiration date. It is assigned a recommended retest date of approximately 3.5 years after the quality release date. A recommended retest date is the period of time during which the product is expected to remain within established stability specifications, provided that it has been stored under defined conditions. After the Retest Date, product samples should be examined to ensure that the product is still in compliance with the established specifications. For more information, you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/US/en/life-science/quality-and-regulatory-management/quality-services.
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Once reconstituted in water, can Doxycycline hyclate be stored long-term at -20°C ?
1 answer-
Aqueous solutions of Doxycycline are light sensitive and should be used within 48 hours. The long term stability of aqueous solutions at -20°C has not been evaluated. However, the Doxycycline HCl Ready Made solution is prepared at 100 mg/ml in DMSO. This product is assigned a shelf life of 1 year and is stored at -20°C. See the links below to review the product datasheet for D9891, as well as a sample Certificate of Analysis for the Ready Made Solution, D3072.
Datasheet, D9891
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/162/885/d9891pis.pdfSample Certificate, D3072
https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/D3072-1ML0000229139.pdfHelpful?
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What type of activity does Product D9891, Doxycycline hyclate, exhibit?
1 answer-
Doxycycline is a broad spectrum antibiotic and bacteriostatic agent against gram positive/negative bacteria and it has antiprotozoal activity.
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At what concentration should I use D9891, Doxyclycline hyclate, for cell culture?
1 answer-
Doxycycline is often used in culture at a concentration of 0.1-12 μg/ml.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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The name of Product D9891 is Doxycycline hyclate. What does hyclate mean?
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Hyclate is the United States Adopted Names (USAN) approved contraction for monohydrochloride hemiethanolate hemihydrate.
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What is the solubility of Doxycycline hyclate, Product D9891?
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Doxycycline hyclate is soluble in water at up to 50 mg/ml.
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