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D2812

Sigma-Aldrich

REDTaq® Genomic DNA Polymerase

without MgCl2

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About This Item

MDL number:
MFCD01635818
UNSPSC Code:
12352204
NACRES:
NA.55

form

liquid

usage

sufficient for 1000 reactions
 sufficient for 250 reactions
 sufficient for 2500 reactions

feature

Difficult Templates/Specialty Enzymes PCR
dNTPs included: no
hotstart: no

concentration

1 unit/μL

technique(s)

PCR: suitable

color

red

input

purified DNA

application(s)

agriculture

shipped in

wet ice

storage temp.

−20°C

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General description

REDTaq® Genomic DNA Polymerase is a unique blend of Taq DNA Polymerase with an inert red dye. This special formulation is designed to provide enhanced amplification of more complex or genomic templates. REDTaq Genomic DNA Polymerase is highly sensitive, produces increased yields and is capable of generating longer product lengths. It has all the advantages of REDTaq DNA polymerase, such as easy visualization of enzyme addition and complete reaction mixing, and direct loading to an agarose gel. The inert red dye does not effect automated or manual sequencing, restriction digestions or other downstream applications. The dye can easily removed by any standard purification method.

Application

REDTaq® Genomic DNA Polymerase has been used as a component of the polymerase chain reaction (PCR) mix for PCR amplification. It has also been used as a component of the preamplification mix for PCR in amplified fragment length polymorphism (AFLP) analysis of Fusarium oxysporum sp. isolates.

Features and Benefits

  • Enhanced amplification on genomic and difficult DNA templates
  • Same great performance as Taq DNA Polymerase in a more convenient format for high throughput applications
  • Quick recognition and confirmation of appropriate mixing
  • No loading buffers or tracking dyes necessary. Sample can be taken directly from reaction and loaded onto an agarose gel
  • The samples can be re-amplified as in nested PCR
  • The red dye migrates faster than bromophenol blue
  • Greater consistency across reactions due to proper mixing

Components

Includes 10× PCR reaction buffer without MgCl2 and a separate tube of 25 mM MgCl2

Unit Definition

One unit incorporates 10 nmol of total dNTPs into acid precipitable DNA in 30 min. at 74°C.

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.
REDTaq is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Paola Persico et al.
Veterinary dermatology, 22(6), 521-527 (2011-06-10)
Ulcerative dermatitis caused by feline herpes virus 1 (FHV-1) is an uncommon disease characterized by cutaneous ulcers secondary to epidermal, adnexal and dermal necrosis. Differential diagnoses for FHV-1 lesions include, but are not limited to, mosquito bite hypersensitivity and eosinophilic
Characterization of Fusarium oxysporum f. sp. radicis-cucumerinum attacking melon under natural conditions in Greece
Vakalounakis DJ
Plant Pathology, 54(3), 339-346 (2005)
Wiesław Bogdanowicz et al.
Proceedings of the National Academy of Sciences of the United States of America, 106(30), 12279-12282 (2009-07-09)
We report the results of mitochondrial and nuclear DNA analyses of skeletal remains exhumed in 2005 at Frombork Cathedral in Poland, that are thought to be those of Nicolaus Copernicus (1473-1543). The analyzed bone remains were found close to the
K L Mealey et al.
Journal of veterinary pharmacology and therapeutics, 38(5), 429-433 (2015-02-11)
The aim of this study was to sequence all exons of the ABCB1 (MDR1) gene in cats that had experienced adverse reactions to P-glycoprotein substrate drugs (phenotyped cats). Eight phenotyped cats were included in the study consisting of eight cats

Protocols

Amplification of Genomic DNA using REDTaq® DNA Polymerase

Reactions using REDTaq® DNA polymerase are formulated as any PCR mixtures. There are no additional reaction preparation steps or protocol changes required.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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