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D1556

Sigma-Aldrich

OptiPrep™ Density Gradient Medium

OptiPrep™ Density Gradient Medium

used for cell and subcellular organelle isolation

Synonym(s):

Iodixanol solution

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250 ML
₹42,661.33

₹42,661.33

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250 ML
₹42,661.33

About This Item

CAS Number:
92339-11-2
MDL number:
MFCD00867965
UNSPSC Code:
12352207
PubChem Substance ID:
329798703
NACRES:
NA.75
Quality Level:
200
form:
liquid
sterility:
sterile
storage temp.:
2-8°C

₹42,661.33

In StockDetails

Request a Bulk Order

Quality Level

200

sterility

sterile

form

liquid

density

1.319-1.321 g/mL

application(s)

hematology
histology

storage temp.

2-8°C

SMILES string

CC(=O)N(CC(O)CN(C(C)=O)c1c(I)c(C(=O)NCC(O)CO)c(I)c(C(=O)NCC(O)CO)c1I)c2c(I)c(C(=O)NCC(O)CO)c(I)c(C(=O)NCC(O)CO)c2I

InChI

1S/C35H44I6N6O15/c1-13(52)46(30-26(38)20(32(59)42-3-15(54)9-48)24(36)21(27(30)39)33(60)43-4-16(55)10-49)7-19(58)8-47(14(2)53)31-28(40)22(34(61)44-5-17(56)11-50)25(37)23(29(31)41)35(62)45-6-18(57)12-51/h15-19,48-51,54-58H,3-12H2,1-2H3,(H,42,59)(H,43,60)(H,44,61)(H,45,62)

InChI key

NBQNWMBBSKPBAY-UHFFFAOYSA-N

General description

OptiPrep™ Density Gradient Medium, also known as iodixanol, is a low osmolarity, non-ionic iodinated density gradient medium. It is a sterile and endotoxin-tested solution that comprises 60% (w/v) iodixanol in water, designed for the in vitro isolation of biological particles. It may be used for fractionation and separation of cell and subcellular organelles.[1]

Application

OptiPrep™ has been used to isolate molecules, cells, and subcellular organelles, including DNA, cytokines, lysosomes, endoplasmic reticulum, peroxisomes, T lymphocytes, and natural killer cells.[1] It has also been used for the following applications:

  • to investigate the efficiency of single-cell encapsulation.[1]
  • the gradient-based isolation of senescent cancer cells.[2]
  • isolation of non-enveloped virion isolation from bronchoalveolar lavage.[3]
  • isolation of lipoproteins and macromolecules
  • purification of virions
  • purification of neuron cells

Features and Benefits

OptiPrep™ has numerous biocompatible properties that make it ideal for use.
  • It is biologically inert and does not interfere with assays for marker enzymes.[1]
  • It is non-ionic, non-toxic, and has low osmotic pressure.
  • It forms spontaneous gradients that are stabler and easier to prepare than sucrose.[2]

Other Notes

60% (w/v) solution of iodixanol in water (sterile).

Legal Information

OptiPrep is a trademark of Serumwerk Bernburg AG

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
BCCM7114
MKCV5254
MKCW0884
MKCX0606
MKCW4446

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Chapter 19: Isolation of Subcellular Organelles and Structures
Uwe Michelsen, Jorg von Hagen
Methods in Enzymology, 463 (2009)
Lysosome-Related Effector Vesicles in T Lymphocytes and NK Cells
Lettau, et al.
Scandinavian Journal of Immunology, 82 (2015)
Eloise Hudry et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 18(1), 44-53 (2009-08-06)
The development of Alzheimer's disease (AD) is closely connected with cholesterol metabolism. Cholesterol increases the production and deposition of amyloid-beta (Abeta) peptides that result in the formation of amyloid plaques, a hallmark of the pathology. In the brain, cholesterol is
Tanner C Fadero et al.
eLife, 6 (2017-09-05)
Iodixanol provides an easy and affordable solution to a problem that has limited resolution and brightness when imaging living samples.
Isolation of senescent cells by iodixanol (OptiPrep) density gradient-based separation
Kristina Kovacovicova, Manlio Vinciguerra
Cell Proliferation, 52 (2019)
View All Related Papers

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1 Review This action will navigate to reviews.

Questions

1–9 of 9 Questions  

  1. What is the method used to determine the density mentioned on the Certificate of Analysis (CoA) for D1556 OptiPrep™ Density Gradient Medium?

    1 answer

    1. Based on the provided details, utilizing a densitometer or pycnometer is recommended.

      Helpful?

  2. What is the molarity of OptiPrep (catalog number D1556)?

    1 answer

    1. The molecular weight of OptiPrep is 1552.2 amu, and its density is 1.320 g/ml. The prepared solution contains 60% OptiPrep. Based on these values, the calculated molarity is 0.706 Molar.

      Helpful?

  3. What is the density of OptiPrep: 60% v/v, w/w, or w/v?

    1 answer

    1. The density of OptiPrep is expressed as weight/volume (w/v). The stock concentration from the bottle is 60%.

      Helpful?

  4. What is the osmolarity of OptiPrep?

    1 answer

    1. According to the supplier information, the osmolarity of OptiPrep is stated to be 170 mOsm with a deviation of +/- 15 mOsm.

      Helpful?

  5. What is the viscosity of the solution?

    1 answer

    1. This product is not tested for viscosity. This item is a 60% solution of Iodixanol in water. However, Iodixanol properties reported by drugfuture.com state that a 50% solution has a viscosity of 8.6 mPa at 37°C.

      Helpful?

  6. Why choose Product D1556, OptiPrep, instead of other density gradients such as Percoll or Histopaque?

    1 answer

    1. Percoll and Histopaque must be removed prior to use through washing.  If Percoll and Histopaque are not removed, these density gradients can be toxic to mammalian cells.  In comparison, OptiPrep has no potential for bioaccumulation and can be considered nontoxic to cells. OptiPrep also has lower viscosity as compared to polysucrose based products such as Histopaque or Ficoll.

      Helpful?

  7. How do I store solutions of Product D1556, OptiPrep™ Density Gradient Medium?

    1 answer

    1. After diluting Product D1556, OptiPrep™ Density Gradient Medium, to the required density, the solution can be stored at 4°C. It should be mixed well before use to make sure it has not settled.

      Helpful?

  8. What is the appearance of Product D1556, OptiPrep?

    1 answer

    1. The solution may be clear to slightly yellow.

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  9. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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Reviews

Active Filters

  1. Atlanta, GA
    • Reviews 4
    • Votes 8
    3 out of 5 stars.

    Expensive OptiPrep

    It worked as OptiPrep from other companies, but it's over-priced, in my opinion.

    Helpful?

    1. Response from MilliporeSigma:

      Thank you for taking the time to leave a review! Our Customer Service department is available to discuss getting the best quality products for your needs at the right price. Please feel free to contact us by visiting https://www.sigmaaldrich.com/support/customer-support and submit a Product Pricing and Availability ticket. We look forward to hearing from you soon!

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