SVCT
94122105, human breast, Epithelial
Synonym(s):
SCTV, SCVT
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About This Item
UNSPSC Code:
41106514
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product name
SVCT, 94122105, from human breast
biological source
human breast
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
Cell Line Origin
Human breast epithelium, SV40 transformed
Cell Line Description
SVCT has been established from human breast epithelial cells being transfected with SV40. The cell line shows an abnormal karyotype and was not tumourigenic in athymic male nude mice. SVCT have been found positive for the expression of keratin 7, 8 and 18 but negative for keratin 19. Epithelial cells, dome formation when confluent.
DNA Profile
Not specified
Culture Medium
DMEM + 2 mM Glutamine + 5μg/ml Hydrocortisone + 10μg/ml Insulin + 10% Fetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:2 to 1:4, i.e., seeding at 2-4x10,000 cells/cm2 using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37 °C.
Other Notes
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Disclaimer
This cell line has special release conditions: All customers are required to complete a Cell line Release Authorisation form.
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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