537-B1-1
Reactivity: Anti-Lymphocytes/endothelium, sheep, 93102701
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About This Item
biological source
Mouse x Mouse Hybridoma
ImmunoDon
BALB/c mouse spleen
Myeloma
Sp2/O
Reactivity
Anti-Lymphocytes/endothelium, sheep
technique(s)
cell culture | mammalian: suitable
isotype
IgM
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse Sp2/O x Mouse BALB/c spleen
Cell Line Description
The antibody is directed against antigen expressed on most sheep lymphocytes and also endothelium. The antigen recognised by 537-B1-1 is down regulated after T cell activation.
Immunogen
Sheep lymphocytes
Culture Medium
IDMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS) (may benefit from IL-6 in medium)
Subculture Routine
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.
Other Notes
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