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Product Name
MOP-8, 92111707
biological source
mouse embryo
growth mode
Adherent
karyotype
Not specified
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse, SV40 Polyoma transformed NIH/3T3 cells
Cell Line Description
MOP-8 was developed by transforming NIH/3T3 cells with a hybrid transcription unit composed of the SV40 early promoter fused to the early region of polyomavirus. The cells should be handled under laboratory containment level 2.
Application
Suitable host for transfection studies, polyoma virus replication studies
Culture Medium
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA, 5% CO2; 37°C.
Other Notes
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