Established from normal embryonal tongue tissue of female cat (Felis catus). Cells support the replication of a wide range of feline viruses. Can undergo 50 population doublings.
Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2 37°C. Maintain at pH 7.1-7.3.
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