I-10

89031605, mouse testis, Epithelial

• UNSPSC Code: 41106514

Properties

• Product Name: I-10, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA., 89031605
• biological source: mouse testis
• packaging: tube of 5 μg 89031605-DNA-5UG; pkg of vial of cells 89031605-1VL
• growth mode: Adherent
• karyotype: 2n = 40, hyperdiploid
• morphology: Epithelial
• products: Steroids
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Mouse BALB/c Leydig cell testicular tumour

Cell Line Description

Derived from a Leydig cell testicular tumour of a BALB/c mouse. The cells secrete steroids; this increases at saturation density. Secretion should be retained for over 50 passages. Exposure to 0.00001M cAMP stimulates secretion 4-5 fold. The major products are progesterone and its derivative 20 α-hydroxy-4-pregnen-3-one. It does not respond to interstitial cell stimulating hormone (ICSH) but is stimulated by cyclic adenosine monophosphate (AMP).

Application

Tumourigenicity studies

Culture Medium

Ham′s F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 i.e. seeding at 6x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.