HL60 15-12

88120805, human blood, Not specified

• UNSPSC Code: 41106514

Properties

• Product Name: HL60 15-12, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA., 88120805
• biological source: human blood
• packaging: tube of 5 μg 88120805-DNA-5UG; pkg of vial of cells 88120805-1VL
• growth mode: Suspension
• karyotype: Not specified
• morphology: Not specified
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human Caucasian promyelocytic leukaemia

Cell Line Description

HL60 15-12 is a variant of HL60 which is capable of differentiating towards either neutrophils or monocytes with treatment of appropriate agents.

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 1.25% Dimethyl Sulphoxide (DMSO).

Subculture Routine

Maintain cultures between 2x100,000 to 1,000,000 cells/ml. NB: The cells will differentiate if the culture medium is allowed to become acidic. 5% CO₂; 37°C. Freeze in 10% glycerol and 90% FBS.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.