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AFRC IAH-CC13

88022403, Reactivity: Anti-CD1, bovine

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About This Item

UNSPSC Code:
41106514

biological source

Mouse x Mouse Hybridoma

ImmunoDon

BALB/c mouse spleen

Myeloma

NS1

Reactivity

Anti-CD1, bovine

technique(s)

cell culture | mammalian: suitable

isotype

IgG3

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Mouse NS1 x Mouse BALB/c spleen

Cell Line Description

The antibody is directed against a thymic differentiation antigen in cattle. The antigen is expressed on 50-60% of bovine thymocytes, but not on peripheral blood mononuclear cells. The antigen recognised is the bovine equivalent of CD1.

Immunogen

Bovine thymocytes

Culture Medium

RPMI 1640 + 2mM glutamine + 1% Hypoxanthine, Thymidine (HT) supplement + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. These cells require mouse macrophage feeder layers upon resuscitation

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

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