MRC-9

85020202, human lung (fetal), Fibroblast

• UNSPSC Code: 41106514
• biological source: human lung (fetal)
• growth mode: Adherent
• karyotype: 2n = 46, diploid
• morphology: Fibroblast
• products: Not specified
• receptors: Not specified

Properties

• Product Name: MRC-9, 85020202
• biological source: human lung (fetal)
• growth mode: Adherent
• karyotype: 2n = 46, diploid
• morphology: Fibroblast
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human Caucasian foetal lung

Cell Line Description

Established from normal lung tissue of a 15 week old female Caucasian foetus. Like MRC-5 the line is susceptible to a wide range of viruses. At least 40 population doublings expected.

Application

Virus studies: rhinoviruses, production of viral vaccines

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 8,11
D16S539: 12
D5S818: 12,13
D7S820: 10,11
THO1: 6,8
TPOX: 11
vWA: 16,17

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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