U-251 MG cell line human

9063001, human brain, Pleomorphic/astrocytoid

• Synonym(s): U-251MG Cells, U251 Cells, U251N Cells
• UNSPSC Code: 41106514

Properties

• product name: U-251 MG cell line human, 09063001
• biological source: human brain
• growth mode: Adherent
• karyotype: 2n = 46
• morphology: Pleomorphic/astrocytoid
• products: Contains GFAP positive cells
• receptors: PDGFR alpha and EGFR. See Nistér M. et al., (1988) & (1991)
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human glioblastoma astrocytoma

Cell Line Description

Derived from a malignant glioblastoma tumour by explant technique. U-251 was formerly distributed as U-373 MG (Sigma product no. 89081403) until short tandem repeat (STR)-PCR profiling confirmed identity with U-251. A new deposit of U-373 MG known as U-373 MG (Uppsala) is now available (Sigma product no. 08061901).Background to the identity query for the cell line U-373 MG: The American Type Culture Collection (ATCC) reported that their stock of U-373 MG had been shown to have differing genetic properties to stock from the originator′s laboratory, and to share similarities with another glioblastoma cell line, U-251. In light of this, ECACC undertook an investigation into the authenticity of its own stock of the U-373 MG cell line. ECACC found similar results to the ATCC i.e. the stock held as U-373 MG was found to be identical by STR-PCR profiling to U-251. The U-373 MG cell line listed under product no. 89081403 has been re-named as ′U-251 (formerly known as U-373 MG)′ and has the new Sigma product no. 09063001.

Application

U-251 MG cell line human has been used:

• for the extraction of cancer stem cells from U-251 human glioblastoma cell line
• to study the mechanism of Pax6 (paired box protein)-associated increase in expression of Dkk3 (Dickkopf 3)
• to study the role of JARID1B (jumonji AT-rich interactive domain 1B) in the pathogenesis of glioma
• to study the therapeutic efficacy of Irinophore C combined with temozolomide in glioblastoma tumor model

DNA Profile

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 10,11
D16S539: 12
D5S818: 11,12
D7S820: 10,12
THO1: 9.3
TPOX: 8
vWA: 16,18

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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