C8034
Anti-Clathrin antibody produced in goat
whole antiserum
Synonym(s):
Anti-CHC, Anti-CHC17, Anti-CLH-17, Anti-CLTCL2, Anti-Hc, Anti-MRD56
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About This Item
MDL number:
UNSPSC Code:
12352203
Recommended Products
biological source
goat
Quality Level
conjugate
unconjugated
antibody form
whole antiserum
antibody product type
primary antibodies
clone
polyclonal
contains
15 mM sodium azide
species reactivity
human
technique(s)
indirect immunofluorescence: 1:20 using human foreskin fibroblasts
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... CLTC(1213)
General description
Clathrin consists of three heavy chains, along with three light chains. The weight of heavy chains varies depending on the source of clathrin. It forms a three-legged structure termed triskelion with three clathrin heavy chains (molecular weight 180,000 daltons per chain) and three non-covalently bound light chains having molecular weights of 30,000-40,000 daltons per chain. Although the term clathrin was originally used to designate the heavy chain component of triskelions, clathrin is now used to refer to the entire triskelion protein molecule.
Clathrin consists of three heavy chains, along with three light chains. The weight of heavy chains varies depending on the source of clathrin. It forms a three-legged structure, termed triskelion with three clathrin heavy chains (molecular weight 180,000 daltons per chain) and three non-covalently bound light chains having molecular weights of 30,000-40,000 daltons per chain. Although the term clathrin was originally used to designate the heavy chain component of triskelions, clathrin is now used to refer to the entire triskelion protein molecule.
Specificity
The product specifically labels the heavy chain band of clathrin by immunoblotting.
Immunogen
clathrin from bovine brain coated vesicles
Application
Anti-Clathrin antibody was used:
- for incubating trophozites (2.105) in a study to determine the role of clathrin in endocytic process of fluid-phase pathway.
- in indirect immunofluorescence staining.
- for immunocytohistochemistry study of human platelets to study the colocalization of adhesive proteins with clathrin in human blood platelets.
Biochem/physiol Actions
Clathrin is the main constituent of the polygonal network that forms the layer of coated vesicles and coated pits. It is widely assumed that coated vesicles mediate the selective transfer of molecules and membrane components between specific membranous organelles within cells. It is a type of protein which plays an important function within the cell, including various types of intracellular transport processes.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Molecular structure, function, and dynamics of clathrin-mediated membrane traffic
Kirchhausen T, et al.
Cold Spring Harbor Perspectives in Biology, 6(5), a016725-a016725 (2014)
S A Morris et al.
The Journal of biological chemistry, 265(6), 3354-3357 (1990-02-25)
Protein kinases which co-purify with clathrin-coated vesicles are known to phosphorylate in vitro the 50-kDa subunit of the HA-II adaptor complex and upon inclusion of polylysine the beta-light chain of clathrin and polypeptides above 100 kDa. Here we relate the
M H Klinger et al.
Cell and tissue research, 279(3), 453-457 (1995-03-01)
Coated membranes and vesicles play an important role in receptor-mediated endocytosis and intracellular trafficking in various cell types, and are also present in blood platelets. Platelets take up certain proteins from the blood plasma, such as von Willebrand factor and
Fernando López-Soto et al.
International journal for parasitology, 39(4), 417-426 (2008-10-14)
Entamoeba histolytica is a parasitic protozoan that produces dysentery and often reaches the liver, leading to abscess formation. Ferritin is an iron-storage protein that is mainly found in liver and spleen in mammals. The liver contains a plentiful source of
M Majeed et al.
Infection and immunity, 59(12), 4465-4472 (1991-12-01)
Immunofluorescence was used to examine the distribution of Chlamydia trachomatis serovars L2 and E, F-actin, and clathrin in infected McCoy and HeLa cells. After incubation at 4 degrees C, C. trachomatis serovar L2 was randomly distributed on the McCoy cell
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