C0817

Monoclonal Anti-HLA-DQ antibody produced in mouse

clone HK19, purified immunoglobulin, buffered aqueous solution

• Synonym(s): Monoclonal Anti-HLA-DQ
• UNSPSC Code: 12352203

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: HK19, monoclonal
• form: buffered aqueous solution
• species reactivity: human
• technique(s): flow cytometry: 5 μL using 1 × 10⁶ cells
• isotype: IgG2b
• UniProt accession no.: P01920
• shipped in: wet ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... HLA-DQB1(3119)

Description

Specificity

Recognizes MHC Class II molecules that are preferentially expressed on the surface of B lymphocytes. The antibody reacts weakly with resting or ConA-activated T cells, monocytes, granulocytes, and erythrocytes.

Immunogen

GM-1500 human tumor cell line.

Application

Monoclonal Anti-HLA-DQ antibody produced in mouse is suitable for flow cytometry at a concentration of 5 μL using 1 × 10⁶ cells.

Biochem/physiol Actions

Human leukocyte antigen (HLA)-DQ is a cell surface receptor type protein found on antigen presenting cells. DQ is a αβ heterodimer of the MHC Class II type. It plays a central role in the immune system and is expressed in antigen presenting cells. HLA-DQ expression in cystic fibrosis CF is associated with inflammation and reduces response to Interferon γ (IFNγ). HLA-DQ acts as alloantibody most frequently associated with the generation of de novo donor-specific antibody (DSA) and antibody-mediated-rejection but does not support organ transplantation.

Target description

HLA-DQ molecules are bimolecular glycoprotein complexes of 29 kDa and 33 kDa components. The HLA-DQ antigens are present on peripheral blood lymphocytes and tonsillar and splenic mononuclear cells.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide