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B5002

Sigma-Aldrich

5-Bromo-2′-deoxyuridine

≥99% (HPLC)

Synonym(s):

5-BrdU, 5-Bromo-1-(2-deoxy-β-D-ribofuranosyl)uracil, 5-Bromouracil deoxyriboside, BUdR

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100 MG
₹6,906.35
250 MG
₹8,724.95
500 MG
₹13,238.98
1 G
₹16,215.85
5 G
₹64,657.73
100 G
₹9,74,877.85

₹6,906.35

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100 MG
₹6,906.35
250 MG
₹8,724.95
500 MG
₹13,238.98
1 G
₹16,215.85
5 G
₹64,657.73
100 G
₹9,74,877.85

About This Item

Empirical Formula (Hill Notation):
C9H11BrN2O5
CAS Number:
59-14-3
Molecular Weight:
307.10
Beilstein:
30395
EC Number:
200-415-9
MDL number:
MFCD00006529
UNSPSC Code:
41106305
PubChem Substance ID:
24278270
NACRES:
NA.51

₹6,906.35

In StockDetails

biological source

synthetic (organic)

Quality Level

200

Assay

≥99% (HPLC)

form

powder

mp

191-194 °C (dec.) (lit.)

solubility

DMSO: 50 mg/mL, clear, colorless to very faintly yellow

storage temp.

−20°C

SMILES string

OC[C@H]1O[C@H](C[C@@H]1O)N2C=C(Br)C(=O)NC2=O

InChI

1S/C9H11BrN2O5/c10-4-2-12(9(16)11-8(4)15)7-1-5(14)6(3-13)17-7/h2,5-7,13-14H,1,3H2,(H,11,15,16)/t5-,6+,7+/m0/s1

InChI key

WOVKYSAHUYNSMH-RRKCRQDMSA-N

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Application

5-Bromo-2′-deoxyuridine (5-BrdU) is a thymidine analogue which is incorporated into DNA. 5-BrdU is routinely and extensively used to measure DNA synthesis and to label dividing cells. Consequently 5-BrdU is used to study cell signaling and other processes that induce cell proliferation.
5-Bromo-2′-deoxyuridine has been used:
  • as a supplement to study the effect of electroconvulsive seizures on hippocampal neurogenesis[1]
  • as a supplement to study the loss-of-gene function in adult zebrafish heart[2]
  • in T lymphocyte proliferation assay{133]

Biochem/physiol Actions

5-Bromo-2′-deoxyuridine (BrdU) is used to analyze cell proliferation,[3] because of its facile incorporation into DNA during the S phase of the cell cycle. BrdU stimulates cellular differentiation and maturation in leukemia cell lines, while it inhibits differentiation of friend erythroleukemia cells.[4] Studies of BrdU incorporation often subsequently use BrdU-specific antibodies with fluorescent tags, for detection of the incorporated BrdU, via such methods as flow cytometry or fluorescence microscopy.
Thymidine analog used as a mutagen in genetic research. Selectively incorporated into cellular DNA during S-phase.

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H340,H361fd

Hazard Classifications

Muta. 1B - Repr. 2

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 2

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
HMBK5031
HMBK2247
HMBK1711
HMBK2710
HMBK4458

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Find documentation for the products that you have recently purchased in the Document Library.

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Mechanisms of Differentiation, Volume 2, 85-85 (1990)
Target Validation in Drug Discovery, 109-109 (2011)
Electroconvulsive seizures increase hippocampal neurogenesis after chronic corticosterone treatment
Hellsten J, et al.
The European Journal of Neuroscience, 16(2), 283-290 (2002)
PEG-PLA nanoparticles facilitate siRNA knockdown in adult zebrafish heart
Diao J, et al.
Developmental Biology, 406, 196-202 (2015)
Francisco Alén et al.
International journal of molecular sciences, 20(14) (2019-07-25)
Mutant mice with respect to the splicing factor Zrsr1 present altered spermatogenesis and infertility. To investigate whether Zrsr1 is involved in the homeostatic control that the hypothalamus exerts over reproductive functions, we first analyzed both differential gene and isoform expression
View All Related Papers

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Questions

1–8 of 8 Questions  

  1. Could specific insights into the use of this product be provided? BrdU was administered in water to mice for up to 2 weeks for an in vivo cell proliferation assay. However, upon staining with an anti-BrdU antibody, no BrdU incorporation was detected in the positive control (small intestine), despite trying various concentrations. Any information on the particular use of this product would be much appreciated

    1 answer

    1. Given the BrdU incorporation into DNA of cells, using the Western blot method for detection would be quite challenging. Typically, the immunohistochemistry method is employed for this purpose. When it comes to the application on small intestine samples, the following citation of B5002 via immunohistochemical detection may be useful for protocol reference:

      Title: "The ubiquitin ligase ITCH coordinates small intestinal epithelial homeostasis by modulating cell proliferation, differentiation, and migration." Published in Differentiation. Jan-Feb 2018;99:51-61. PMID: 29309986

      Helpful?

  2. How long can BrdU be stored at -20 degrees and -4 degrees, after being diluted in sterile saline and being filtered with a 0.22 µm filter?

    1 answer

    1. BrdU should be stored at –20 oC and desiccated. Stock solutions can be made in DMSO to up to 50 mg/mL, as indicated in the Product information sheet. The stock can be stored for 6 months at -20°C in single use aliquots.

      No information is available on storage conditions for BrdU dissolved in saline.

      Helpful?

  3. What can I use to detect BrdU, Product B5002 in vivo and in vitro?

    1 answer

    1. For the immunohistochemical detection of BrdU, we recommend Product No. B2531, Monoclonal Anti-BrdU antibody produced in mouse.

      Helpful?

  4. How does BrdU, Product B5002, work as a Thymidine analog?

    1 answer

    1. BrdU replaces Thymidine during the S-phase of cell division.

      Helpful?

  5. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  6. How long do I treat cells with Product B5002, BrdU to incorporate it into the DNA?

    1 answer

    1. Actively growing cells may be treated with 10 μm BrdU for 1 hour.

      Helpful?

  7. What are the hazards associated with BrdU, Product B5002?

    1 answer

    1. BrdU is a known carcinogen and caution should be exercised when handling.  Click on the MSDS hyperlink located on the product detail page for Product No. B5002, 5-Bromo-2´-deoxyuridine.

      Helpful?

  8. What is the stability of Product B5002, BrdU?

    1 answer

    1. BrdU is stable as a powder, kept in the freezer and has a recommended re-test date of two years according to the Product Specification Sheet for Product No. B5002.  Please consult our Product Dating Information Statement for more information on re-test dated products.

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