The suitability of this product for ELISA has not been tested in-house and is likely based on literature or customer feedback. Therefore, specific recommendations for its use in detecting IL-6 levels in rat serum samples using ELISA cannot be provided. It is advised to consult the literature or conduct empirical testing to determine the appropriate usage in this application.
A2153
Bovine Serum Albumin
Bovine Serum Albumin
lyophilized powder, ≥96% (agarose gel electrophoresis)
Synonym(s):
Albumin bovine serum, BSA, Bovine albumin
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About This Item
To inquire about this product A2153, please contact your local Merck office or dealer. Contact Technical Service
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biological source
bovine
Quality Level
Assay
≥96% (agarose gel electrophoresis)
form
lyophilized powder
mol wt
~66 kDa
purified by
cold ethanol fractionation
packaging
poly bottle of
origin
USA origin
technique(s)
ELISA: suitable
blood typing: suitable
microbiological culture: suitable
impurities
≤6.0% Loss on Drying
loss
≤6%
pH
6.5-7.5 (1% in 0.15 M sodium chloride)
7
solubility
water: soluble (40 mg/ml)
UniProt accession no.
foreign activity
BT Virus, none detected
VSV Virus, none detected
storage temp.
2-8°C
Gene Information
bovine ... ALB(280717)
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General description
Bovine Serum Albumin (BSA) is a globular, non-glycosylated α-helical plasma protein produced in the bovine liver. It consists of three domains (I, II, and III), each with two sub-domains (A and B), arranged in a heart-shaped structure.
Application
Bovine serum albumin has been used-
- along with BTS (Beltsville thawing solution), for the extension of sperms during sperm motion analysis[1]
- as a component of modified Krebs-Ringer buffer (dmKRBT)[2]
- in gel shift analysis[3]
- as a blocking agent in immunocytochemistry, immunohistochemistry,(4) immunofluorescence protocol and sandwich enzyme-linked immunosorbent assay (ELISA)
- as a component of phosphate buffer saline (PBS) solution to resuspend cells for analysis during flow cytometry
- as a protein standard to quantify proteins in tissue samples via Bradford’s method
- to measure the enzyme activity of 17β HSD enzyme
Biochem/physiol Actions
Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.
Preparation Note
Often referred to as Cohn fraction V; this product is prepared by a modified method of the Cohn cold ethanol fractionation method.
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.
also commonly purchased with this product
Product No.
Description
Pricing
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Simple histochemical stain for acrosomes on sperm from several species.
Larson JL and Miller DJ
Molecular Reproduction and Development, 52(4), 445-449 (1999)
Bartolomeo Della Ventura et al.
PloS one, 12(2), e0171754-e0171754 (2017-02-10)
Biosensor-based detection provides a rapid and low-cost alternative to conventional analytical methods for revealing the presence of the contaminants in water as well as solid matrices. Although important to be detected, small analytes (few hundreds of Daltons) are an issue
Mateusz Majda et al.
Developmental cell, 43(3), 290-304 (2017-11-08)
The epidermis of aerial plant organs is thought to be limiting for growth, because it acts as a continuous load-bearing layer, resisting tension. Leaf epidermis contains jigsaw puzzle piece-shaped pavement cells whose shape has been proposed to be a result
Determination of intracellular reactive oxygen species and high mitochondrial membrane potential in Percoll-treated viable boar sperm using fluorescence-activated flow cytometry.
Guthrie HD and Welch GR
Journal of Animal Science, 84(8), 2089-2100 (2006)
Maleeha F Ahmad et al.
Arteriosclerosis, thrombosis, and vascular biology, 39(2), 188-199 (2018-12-21)
Objective- Perivascular adipose tissue (PVAT) contains an independent adrenergic system that can take up, metabolize, release, and potentially synthesize the vasoactive catecholamine norepinephrine. Norepinephrine has been detected in PVAT, but the mechanism of its protection within this tissue is unknown.
Related Content
Learn about bovine serum albumin (BSA) structure, molecular weight, function, solubility, how BSA is made, purification methods, applications and purchasing.
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As mentioned on the product page, it is suitable for ELISA, so can it be used for detecting IL-6 levels in rat serum samples using ELISA? If yes, what percentage will be ideal for the assay, and how will the working concentration be prepared?
1 answer-
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Does this BSA contain any lipids?
1 answer-
This product is not tested for lipid content. There are several fatty acid-free options, however, there is no lipid-free albumin product available.
To discuss this further, please contact the Technical Service team who can assist you further. We kindly ask you to navigate to the link https://www.sigmaaldrich.com/techservice and click on "Product Technical Inquires" under the Products Section with all the required information so that a member of our team can reach out to you to assist further. Thank you.
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Is this BSA acetylated?
1 answer-
This product is not acetylated. This product is prepared by a modified method of the Cohn cold ethanol fractionation method.
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Can this product be used as protein standards in BCA assay?
1 answer-
It can be used as a protein standard.
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Product A2153, Albumin from bovine serum (BSA), powder looks tan in color. Is this OK?
1 answer-
The tan color is within specifications. The color can vary because of factors such as concentration of a solution at the time of lyophilization.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Is Product A2153, Albumin from bovine serum (BSA), suitable for use in immunoblotting experiments as a blocking agent?
1 answer-
We have not tested A2153 in quality control for use in immunoblotting applications as a blocking agent. Because it contains globulins, we would generally not recommend A2153 for use as a blocking agent.
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Is Product A2153, Albumin from bovine serum (BSA), suitable for cell culture?
1 answer-
We do not test A2153 for cell culture use, or for any specifications such as endotoxin content. Customers will have to exercise their own judgment as to whether they wish to use A2153 in cell culture, or can search the literature for publications that cite this product number.
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Is Product A2153, Albumin from bovine serum (BSA), suitable for use in ELISA?
1 answer-
We have not tested A2153 in quality control for use in ELISA applications. Because it contains globulins, we would generally not recommend A2153 for use in ELISA.
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