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17490

Sigma-Aldrich

Bromocresol Purple

for microscopy (Hist., Vit.), indicator (pH 5.2-6.8)

Synonym(s):

5,5′-Dibromo-o-cresolsulfonphthalein, Bromcresol purple sultone form

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About This Item

Empirical Formula (Hill Notation):
C21H16Br2O5S
CAS Number:
115-40-2
Molecular Weight:
540.22
Beilstein:
359618
EC Number:
204-087-8
MDL number:
MFCD00011681
UNSPSC Code:
12171500
PubChem Substance ID:
57647609
NACRES:
NA.47

grade

for microscopy (Hist., Vit.)
indicator (pH 5.2-6.8)

form

powder

technique(s)

titration: suitable

pH

5.2-6.8, yellow to purple

mp

240 °C (dec.) (lit.)

solubility

hot ethanol: 0.1 g/10 mL

εmax

≥400 at 414-424 nm in methanol

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

Cc1cc(cc(Br)c1O)C2(OS(=O)(=O)c3ccccc23)c4cc(C)c(O)c(Br)c4

InChI

1S/C21H16Br2O5S/c1-11-7-13(9-16(22)19(11)24)21(14-8-12(2)20(25)17(23)10-14)15-5-3-4-6-18(15)29(26,27)28-21/h3-10,24-25H,1-2H3

InChI key

ABIUHPWEYMSGSR-UHFFFAOYSA-N

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General description

Bromocresol purple is a pH indicator, which changes color from yellow (at low pH 5.2) to violet (above pH 6.8). It aids in determining the dead cell count for cells with plasma membrane damage. Bromocresol purple is widely used as a fluorescent stain in yeast cells. Cells with an intact plasma membrane are not stained, while damaged cells are seen as blue-grey ghosts.

Application

Spectrophotometric determination of serum-albumin

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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Fluorescent staining with bromocresol purple: a rapid method for determining yeast cell dead count developed as an assay of killer toxin activity
Kurzweilova H and Sigler K
Yeast, 9(11), 1207-1211 (1993)
Yeast colonies synchronise their growth and development
Palkova Z and Forstova J
Journal of Cell Science, 113(11), 1923-1928 (2000)
Faizah Mohd Faizul et al.
Journal of photochemistry and photobiology. B, Biology, 90(1), 1-7 (2007-11-21)
The interaction between bromocresol purple (BCP) and bovine serum albumin (BSA)/porcine serum albumin (PSA) was investigated both in the absence and presence of bilirubin (BR) using absorption/absorption difference spectroscopy. A significant red shift in the absorption maxima of BCP accompanied
Annalise E Zemlin et al.
Nutrition (Burbank, Los Angeles County, Calif.), 25(10), 1006-1010 (2009-06-03)
Hypoalbuminemia is known to occur in critically ill patients and is associated with increased mortality. Severe hypoalbuminemia is defined in the literature as serum albumin levels lower than 24 g/L. Albumin levels were measured in our laboratory using the bromocresol
André Klauss et al.
Photosynthesis research, 102(2-3), 499-509 (2009-03-31)
Determination of thermodynamic parameters of water oxidation at the photosystem II (PSII) manganese complex is a major challenge. Photothermal beam deflection (PBD) spectroscopy determines enthalpy changes (ΔH) and apparent volume changes which are coupled with electron transfer in the S-state
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