14562
2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein tetrakis(acetoxymethyl) ester
BioReagent, for fluorescence
Synonym(s):
BCECF-AM
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1 MG
₹23,598.50
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1 MG
₹23,598.50
About This Item
Empirical Formula (Hill Notation):
C39H36O19
Molecular Weight:
808.69
MDL number:
UNSPSC Code:
12352108
NACRES:
NA.32
Recommended Products
grade
for fluorescence
product line
BioReagent
form
solid
solubility
DMF: soluble
DMSO: soluble
acetonitrile: soluble
fluorescence
λex 482 nm; λem 528 nm in 0.1 M Tris pH 8.0 (esterase)
suitability
suitable for fluorescence
storage temp.
2-8°C
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Application
An uncharged, nonfluorescent molecule that is useful for noninvasive bulk loading of cell suspension because it is capable of permeating the cell membrane. Cellular nonspecific esterases cleave its lipophilic blocking groups, resulting in a charged, fluorescent form of the molecule. Compared to the parent compound, this charged derivative leaks out of the cell at a much slower rate. Utilized for pH measurements in perfused tissues[1][2][3], intercellular spaces[4][5], mammalian cells[6][7][8], plant cells[9], bacteria[10][11], and yeast[12][13]. Also, employed to investigate in assays for cellular functional properties such as adhesion[14][15][16][17][18][19], multi-drug resistance[20][21][22], viability and cytotoxicity[23][24][25][26][27], apoptosis[28] and chemotaxis[15].
Cell-permeable ester of BCECF that, on hydrolysis by cytosolic esterases, yields the intracellularly trapped pH-indicator BCECF. Allows simultaneous recording of cell volume changes and intracellular pH in single osteosarcoma cells.
Packaging
Bottomless glass bottle. Contents are inside inserted fused cone.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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J T Seo et al.
Pflugers Archiv : European journal of physiology, 426(1-2), 75-82 (1994-01-01)
Intracellular pH (pHi) has been measured in intact, perfused rat mandibular salivary glands loaded with the fluorescent pH indicator BCECF [2',7'-bis(2-carboxyethyl)-5(6)- carboxyfluorescein]. Glands mounted in the cuvette of a conventional bench-top spectro-fluorometer were perfused for 5 min with the acetoxymethyl
J S Leeder et al.
Analytical biochemistry, 177(2), 364-372 (1989-03-01)
Studies of drug toxicity, toxicologic structure-function relationships, screening of idiosyncratic drug reactions, and a variety of cytotoxic events and cellular functions in immunology and cell biology require the sensitive and rapid processing of often large numbers of cell samples. This
L Homolya et al.
The Journal of biological chemistry, 268(29), 21493-21496 (1993-10-15)
In this report we show that NIH-3T3 mouse fibroblasts stably expressing the human multidrug transporter (MDR1 or P-glycoprotein), in contrast to the control NIH-3T3 cells, actively extrude the hydrophobic acetoxymethyl ester (AM) derivatives used for cellular loading of various fluorescent
S Goelz et al.
The Journal of biological chemistry, 269(2), 1033-1040 (1994-01-14)
The mammalian cDNA encoding alpha (1,3)-fucosyltransferase (alpha (1,3)Fuc-T) termed ELAM-1 ligand fucosyltransferase (ELFT) or Fuc-TIV was previously cloned by three groups who reported different results from transfection studies Goelz et al. (Goelz, S. E., Hession, C., Goff, D., Griffiths, B.
J R Sellers et al.
Journal of immunological methods, 172(2), 255-264 (1994-06-24)
A cytotoxicity assay has been developed based on the measurement of the proliferative activity of surviving cells as quantified by a cell-incorporated fluorescent dye, 2',7'-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF). The BCECF proliferative assay is fast (the results are obtained within 3-4 days depending
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