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06713

Sigma-Aldrich

Atto 532 DMPE

suitable for fluorescence

Synonym(s):

1,2-Dimyristoyl-sn-glycero-3-phosphoethanolamine labeled with Atto 532

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

Assay

≥90.0% (HPCE)

manufacturer/tradename

ATTO-TEC GmbH

fluorescence

λex 537 nm; λem 559 nm±5 nm in ethanol

suitability

suitable for fluorescence

storage temp.

−20°C

General description

Atto 532 is a fluorescent label related to the well-known dye Rhodamine 6G. Characteristic features of the label are strong absorption, high fluorescence quantum yield, high photostability, and excellent water solubility. Thus Atto 532 is highly suitable for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, STED etc. Additionally the dye highly qualifies to be applied in flow cytometry (FACS), fluorescence in-situ hybridization (FISH) and many more. The fluorescence is excited most efficiently in the range 515 - 545 nm.
A suitable excitation source for Atto 532 is the 532 nm output of the frequency-doubled Nd:YAG laser.

Atto-Dye Labeled Phospholipids
Sigma-Aldrich offers a variety of glycero-phospholipids carrying one or two fatty acid groups (lipophilic groups) and a phosphate ester residue (hydrophilic group). They are labeled at the hydrophilic head group. After incorporation of the phospholipid into a membrane the fluorophore is located at the water/lipid interface of the membrane. We currently provide 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine (DPPE), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE), palmitoyl-sn-glycero-phosphoethanolamine (PPE), and 1,2-dimyristoyl-sn-glycero-3-phospho-ethanolamine (DMPE) labeled with Atto-dyes.

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Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Technical Review. Types of Imaging-Direct STORM.
Jensen, E.; Crossman, D. J.
The Anatomical Record, 297(12), 2227-2231 (2014)
Breaking the diffraction barrier in fluorescence microscopy at low light intensities by using reversibly photoswitchable proteins.
Hofmann, M.; et al.
Proceedings of the National Academy of Sciences of the USA, 102(49), 17565?17569-17565?17569 (2005)
Fluorescence lifetime imaging with pulsed diode laser enabled stimulated emission.
Ge, J.; Kuang, C.; Lee, SS.; Kao, FJ
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Fluoreszierende Rhodamine und fluorogene Carbopyronine fur die STED-Mikroskopie lebender Zellen.
Butkevich, Alexey N.; et al.
Angewandte Chemie (International Edition in English), 128(10), 3350-3355 (2016)
Analysis of replication factories in human cells by super-resolution light microscopy.
Cseresnyes, Z.; et al.
BMC Cell Biology, 10(1), 88-88 (2009)

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