This product is pooled from a bovine source. The bile salt mixture is composed of approximately 50% each of sodium deoxycholate and sodium cholate. The exact composition is not tested. The manufacturing process is consistent, however, given the nature of the source, some variability between lots is expected. The degree of variability does not negatively impact microbial growth. Please see section 3 of the Safety Data Sheet:
https://www.sigmaaldrich.com/sds/sial/b3426
B3426
Bile salts mixture
suitable for microbiology
Synonym(s):
Bile acids sodium salt, cholic acid-deoxycholic acid sodium salt mixture
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100 G
₹32,944.80
About This Item
UNSPSC Code:
41106212
NACRES:
NA.74
Recommended Products
sterility
non-sterile
Quality Level
form
powder
packaging
pkg of 100 g
loss
≤6% loss on drying
color
yellow to brownish-yellow
pH
7±0.5
solubility
distilled water: freely soluble
application(s)
microbiology
storage temp.
10-30°C
General description
Bile salts mixture is a combination of an organic sodium salt with a conjugate of any of the bile acids along with either glycine or taurine. Bile salts are composed of four different kinds of bile acids - cholic, deoxycholic, chenodeoxycholic, and lithocholic acids. Bile salts are extracted under controlled conditions from purified fresh bile, to be used in bacteriological culture media, where it behaves as a selective inhibitory agent that inhibits Gram-positive bacteria and induced germination. It also inhibits the outgrowth of the germinated spores but does not interfere with the growth of Enterobacteriaceae.
Application
Bile salts mixture is recommended in diverse media like MacConkey Agar, SS Agar, and Violet Red Bile Agar. It increases the selectivity of the media to Enterobacteriaceae by inhibiting Gram-positive bacteria. It is used as a selective agent in culture media for the selective growth of intestinal bacteria like E. coli, other fecal coliforms, and fecal Streptococci. But it is also used as a selective agent in media for the detection of Yersinia enterocolitica, Aeromonas hydrophila, and Vibrio species.
Preparation Note
Extracted from purified, fresh bile.
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 3
Storage Class Code
11 - Combustible Solids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Chhatra B Kunwar et al.
Microbiology (Reading, England), 166(12), 1149-1159 (2020-11-19)
Colonization of the gastrointestinal tract by enterohaemorrhagic Escherichia coli (EHEC) is critically dependent on its ability to sense and respond to various microenvironments within the host. EHEC exposure to physiologically relevant levels of bile salts upregulates the two-component system, pmrAB
Crystal Gadishaw-Lue et al.
Infection and immunity, 89(2) (2020-11-25)
During passage through the human gastrointestinal tract, enterohemorrhagic Escherichia coli (EHEC) is exposed to membrane-damaging bile in the small intestine. We previously reported that EHEC treatment with a physiological bile salt mixture upregulates basRS, encoding a two-component system, and arnBCADTEF
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What us the bile salt composition of this mixture? Is this constant between batches?
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