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A7002

Sigma-Aldrich

Agar

ash 2.0-4.5%

Synonym(s):

Agar-agar, Gum agar

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About This Item

Linear Formula:
(C12H18O9)n
CAS Number:
EC Number:
MDL number:
UNSPSC Code:
50405901
eCl@ss:
42040102
NACRES:
NB.22

biological source

algae (Rhodophyceae)

Quality Level

form

flakes
powder or crystals

technique(s)

tissue culture: suitable

ash

2.0-4.5%

gel strength

600-1200 g/cm2 (1.5%, 20 oC)

InChI

1S/C14H24O9/c1-5-8(16)13-11(7(21-5)4-20-13)23-14-10(18)12(19-2)9(17)6(3-15)22-14/h5-18H,3-4H2,1-2H3/t5?,6-,7?,8-,9+,10-,11?,12+,13+,14?/m1/s1

InChI key

GYYDPBCUIJTIBM-DYOGSRDZSA-N

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General description

Agar is a polysaccharide consisting of β- D-galactopyranosyl and 3,6-anhydro-α-L- galactopyranosyl units. Depending on the source agar exists as various substituted forms such as methyl and sulphate ether. It is a linear galactan polysaccharide isolated from the cell wall of Rhodophyceae class of seaweeds.

Application

Agar has been used:
  • as a component of solid N6 complete medium
  • in modified Murashige and Skoog (MS) medium for culturing germinating lentil and faba bean seeds and in the regeneration of transgenic cauliflower and broccoli explants
  • as a component of agar mixture to simulate a vascular phantom in carotid vessel

Biochem/physiol Actions

Agar exists in left-handed helical conformation and aggregation of hexagonal fibers leads to gel formation. The hydrophobicity of the disaccharides determines its gelling property and solubility. Agar is a major component of the microbial solid medium. It has applications in tissue engineering, pharmaceutics and in cell encapsulation.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Successful preparation and characterization of biotechnological grade agarose from indigenous Gelidium amansii of Taiwan
Wang TP, et al.
Process Biochemistry (Oxford, United Kingdom), 47(3), 550-554 (2012)
Plant growth regulators improve in vitro flowering and rapid generation advancement in lentil and faba bean
Mobini SH, et al.
In Vitro Cellular & Developmental Biology, 51(1), 71-79 (2015)
A Method for Radioactive labelling of Hebeloma cylindrosporum to study plant-fungus interactions
Becquer A, et al.
Bio-protocol, 7(20), 550-554 (2017)
Recovery of phenotypically normal transgenic plants of Brassica oleracea upon Agrobacterium rhizogenes-mediated co-transformation and selection of transformed hairy roots by GUS assay
Puddephat IJ, et al.
Molecular breeding : new strategies in plant improvement, 7(3), 229-242 (2001)
Chemical structure and physico-chemical properties of agar
International Workshop on Gelidium, 21(11), 137-148 (1991)

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