70184

Antibiotic Broth

suitable for microbiology, NutriSelect^® Plus

• Synonym(s): Antibiotic Medium No. 3, Assay Broth, Penassay Broth, Grove and Randall Antibiotic Broth
• UNSPSC Code: 41171504
• NACRES: NA.74

Properties

• sterility: non-sterile
• Quality Level: 200
• product line: BioChemika
• form: powder
• shelf life: limited shelf life, expiry date on the label
• composition: dextrose, 1.0 g/L ; dipotassium hydrogen phosphate, 3.68 g/L ; meat extract, 1.5 g/L ; peptone (from meat), 5.0 g/L ; potassium dihydrogen phosphate, 1.32 g/L ; sodium chloride, 3.5 g/L ; yeast extract, 1.5 g/L
• manufacturer/tradename: NutriSelect^® Plus
• technique(s): microbe id | susceptibility testing: suitable; microbiological culture: suitable
• final pH: 7.4±0.2 (25 °C)
• application(s): clinical testing; food and beverages; life science and biopharma; pharmaceutical; microbiology
• suitability: bacteria; nonselective for Escherichia coli; nonselective for Klebsiella spp.; nonselective for Staphylococcus spp.; nonselective for Streptococcus spp.; nonselective for coliforms

Description

Application

Used in antibiotic assay work (USP, FDA, Ph Eur) according to Grove & Randall; used in the turbidimetric assay of e.g. penicillin, bacitracin and kanamycin with S. aureus and for the cylinder assay of e.g. erythromycin and chloramphenicol with M. inteus.

Preparation Note

Dissolve 17.5 g in 1 litre of warm (60 C) distilled water and sterilize by autoclaving at 121°C for 15 minutes.

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves