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11717472001

Roche

Hybridization Buffer

pH 6.5-6.7 (25 °C), suitable for hybridization, solution, pkg of 100 mL

Synonym(s):

Buffer

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About This Item

UNSPSC Code:
41105326

form

solution

Quality Level

100

packaging

pkg of 100 mL

manufacturer/tradename

Roche

technique(s)

hybridization: suitable

pH

6.5-6.7 (25 °C)

storage temp.

2-8°C

Related Categories

General description

Hybridization Buffer is a denaturing solution that optimizes the binding of DNA and RNA probes to specific target sequences in various techniques.
Hybridization buffer is used in combination with the PCR ELISA (enzyme-linked immunosorbent assay) and the series of DIG-Detection ELISAs for nucleic-acid hybridization reactions.

Application

Hybridization Buffer has been used for the prehybridization of coverslips in protein competition assay of human foreskin fibroblasts (HFF) cells. It has also been used to perform hybridization in northern blotting.

Physical form

Solution

Other Notes

For life science research only. Not for use in diagnostic procedures.

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H317

Hazard Classifications

Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

No data available

Flash Point(C)

No data available

Certificates of Analysis (COA)

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Digoxigenin (DIG) Labeling Methods

Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.

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