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Key Documents

10481238001

Roche

pBR322 DNA

solution, pkg of 200 μL (250 μg/ml)

Synonym(s):

dna, pbr 322

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About This Item

UNSPSC Code:
41106610

form

solution

packaging

pkg of 200 μL (250 μg/ml)

manufacturer/tradename

Roche

storage condition

avoid repeated freeze/thaw cycles

storage temp.

−20°C (−15°C to −25°C)

General description

Plasmid for cloning of double-digested restriction fragments.

Application

Select for recombinant DNA. The Pst I and Pvu I cleavage sites in the ampicillin gene, or the Cla I, Hind III, BamH I, and Sal I sites in the tetracycline gene allow the insertion of foreign DNA fragments, and inactivation of one of these genes. pBR322 DNA has been used in to study artificial metallonuclease activity.

Sequence

Chain Length 4,361 bp

Physical form

Solution, 250 μg/ml, in 10 mM Tris-HCl, 1 mM EDTA, pH 8.0, ready-to-use

Storage and Stability

Store at -15 to -25°C until use; once thawed, store at 2-8 °C.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

No data available

Flash Point(C)

No data available


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Andreea Prisecaru et al.
Journal of medicinal chemistry, 56(21), 8599-8615 (2013-10-18)
The synthetic chemical nuclease, [Cu(1,10-phenanthroline)2](2+), has stimulated research within metallonuclease development and in the area of cytotoxic metallodrug design. Our analysis reveals, however, that this agent is "promiscuous" as it binds both dsDNA and protein biomolecules, without specificity, and induces

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