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UFC9100

Millipore

Amicon® Ultra Centrifugal Filter, 100 kDa MWCO

sample volume 15 mL, regenerated cellulose membrane, MWCO 100 kDa

Synonym(s):

100 kDa MWCO, 15 mL sample volume, Centrifugal concentrator

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8 PKG
₹18,220.01
24 PKG
₹47,670.00
96 PKG
₹1,87,940.01

₹18,220.01

Estimated to ship on20 February 2025Details

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8 PKG
₹18,220.01
24 PKG
₹47,670.00
96 PKG
₹1,87,940.01

About This Item

UNSPSC Code:
41104916
eCl@ss:
36100101
NACRES:
NB.24
material:
polyethylene cap
polyethylene liner
polypropylene tube
regenerated cellulose membrane
regenerated cellulose support
styrene-butadiene housing
pore size:
100 kDa MWCO
L:
12.1 cm (4.807 in.)
diam.:
2.97 cm
filtration area:
7.6 cm2
volume:
15 mL
sterility:
non-sterile

₹18,220.01

Estimated to ship on20 February 2025Details

Request a Bulk Order

material

polyethylene cap
polyethylene liner
polypropylene tube
regenerated cellulose membrane
regenerated cellulose support
styrene-butadiene housing

Quality Level

400

sterility

non-sterile

product line

Amicon®

manufacturer/tradename

Amicon® Ultra

parameter

15 mL sample volume

technique(s)

protein extraction: suitable
protein purification: suitable

L

12.1 cm (4.807 in.)

diam.

2.97 cm

filtration area

7.6 cm2

volume

15 mL

pore size

100 kDa MWCO

shipped in

ambient

Related Categories

General description

Amicon® Ultra 15 mL centrifugal devices with Ultracel® 100,000 Dalton molecular weight cutoff filters provide fast ultrafiltration, and reliable retention of macromolecules. For example, IgG (156 kDa) at 1 mg/ml initial concentration is retained at >90% .

The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 80-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss.

The concentrate is collected from the filter device sample reservoir using a pipetter, while the ultrafiltrate is collected in the provided centrifuge tube. The device can be spun in a swinging bucket or fixed angle rotor. With their vertical membrane design and deadstop, Amicon® Ultra 15 mL filters deliver the highest recovery with short spin times.

Application

  • Ultrafiltration with 100,000 Dalton molecular weight cutoff Ultracel® membrane.
  • Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms.
  • Purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLS.
  • Concentration of dilute or pre-purified proteins from column eluents.
  • Desalting, buffer exchange and protein dialysis.

Features and Benefits

  • Ultracel® regenerated cellulose membrane with 100 kDa NMWCO delivers retentate recovery of greater than 90%.
  • Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 10–15 minutes).
  • Heat-sealed membrane minimizes downstream extractables.
  • 100% integrity tested for reliable performance.
  • Convenient sample monitoring with translucent housing and volume gradations.
  • Direct pipettor sample access eliminates processing step to recover concentrate.
  • High concentration factors of 80–100X.

Legal Information

Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany

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Questions

1–10 of 12 Questions  

  1. Hello, Are these tubes valid for AAV concentration? And if so, are these valid for all serotypes? Also, the user guide says that the membrane's material is 'Ultracel® low binding regenerated cellulose'. What's the difference with the PES membrane?

    1 answer

    1. These devices can be used for virus concentration but are not validated for any specific type. Both PES and regenerated cellulose membranes have low binding, but PES has a faster flow rate. For more information, please refer to the technical article on the website: https://www.sigmaaldrich.com/technical-documents/technical-article/analytical-chemistry/filtration/viral-concentration-amicon-ultrafiltration

      Helpful?

  2. Hi, I have plan to use the Amicon Ultra-15 100K for Exosome extraction from cell culture supernatant, could you please let me know what would be the best speed (rpm/rcf) and time for doing that? Thanks

    1 answer

    1. Typically, Amicon-15 devices are spun at 4,000 x g (relative centrifugal force) for exosome isolation. However, spin times may need to be optimized, based on the sample type and volume. The image below demonstrated the relationship between volume and spin times. Please see the link below to review the product User Guide. More specific spin time information is available on page 3.
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/251/395/pr05035w-ug-ms.pdf

      Helpful?

  3. What is the minimum starting volume I can load on Ultra 100K (100,000 MWCO) devices? Can I load as little as 500 µL?

    1 answer

    1. This product is intended for use with 4-15 mL initial sample volumes. The device has not been evaluated for use with volumes smaller than those recommended. While it may be possible to concentrate samples as low as 500 uL, the recovery volume cannot be predicted. Please see the link below to review an alternative Amicon device specifically designed for 500 uL samples.
      https://www.sigmaaldrich.com/product/mm/ufc5100

      Please see the following link to review the full list of 100 kDa MWCO Amicon Ultra products:
      https://www.sigmaaldrich.com/substance/amiconultracentrifugalfilter100kdamwco1234598765

      Helpful?

  4. Does the Amicon Ultra has residues that may released into the concentrated product', like Beta-Glucan?

    1 answer

    1. These devices contain trace amounts of glycerine. If this material interferes with downstream analysis, rinse the device with buffer or Milli-Q® water prior to use. Should interference continue, rinse with 0.1 N NaOH followed by a second spin of buffer or Milli-Q® water. NOTE: Do not allow the membrane to dry out following rinse steps. If the product will not be used immediately after rinsing, leave the final buffer or water on the membrane until the usage. Please see the link below to review the full product User Guide:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/251/395/pr05035w-ug-ms.pdf

      Helpful?

  5. I just noticed that Amicon ultra - 15 centrifugal filters are non-sterile. Has this been always the case? REF: UFC910024, LOT:0000193199

    1 answer

    1. The Amicon Ultra devices have always been non-sterile. These devices cannot be sterilized by UV or autoclaving. However, it is possible to sanitize the devices with 70% ethanol. Please see the protocol for sanitization below:

      1. Reagent preparation: 70% Ethanol solution (v/v) is prepared by dilution in distilled water. Both the ethanol solution and distilled water used for rinsing should be filter-sterilized using sterile, 0.22 µm sterilizing grade filter devices, such as the 50 mL Steriflip® device SCGP00525 or SE1M179M6.

      2.Sanitization Protocol:

      A. Fill the Amicon® Ultra concentrator device to its published capacity with filter-sterilized 70% Ethanol, and cap the unit (note that the collection tubes can be sanitized in the same manner).
      B. Invert device by hand or in an end-over-end mixer to continuously wet all internal surfaces of the device.
      C. Incubate for a minimum of 5 minutes (note that the device can be capped and left for up to 24 hours if it is not going to be used immediately).
      D. Spin at recommended G-force using appropriate centrifuge until at least 50% of the volume has passed through the device.
      E. Aseptically discard any remaining ethanol from the filter device and filter collection tube.
      F. Immediately rinse away residual ethanol by adding the filter-sterilized water to the device's published capacity, mix gently to wet all areas, and centrifuge until at least 50% of the volume has passed through the device.
      G. Discard any remaining liquid from both the concentrator device and filter collection tube before use.

      DO NOT let the filter membrane dry out in concentrator unit. If the devices are not to be used immediately, fill the devices with filter-sterilized distilled water, cover, and store refrigerated. Remove liquid from device as previously described prior to use.

      Helpful?

  6. What is the maximum volume that can be loaded into the Amicon 15 device when using both fixed angle and swinging bucket rotor centrifuges?

    1 answer

    1. When using the fixed angle centrifuges with the Amicon 15 device, the maximum volume that can be loaded is 12 mL. It is important to place the device at a 35-degree angle. On the other hand, when using swinging bucket centrifuges, the maximum volume that can be loaded into the Amicon 15 device is 15 mL.

      Helpful?

  7. Hi, how should we place the amicon ultra-15 tube in the fixed-angle rotor, (could you please explain the sentence "When using a fixed-angle rotor, orient the device with the membrane panel facing up" , sorry I'm not a native English speaker

    1 answer

    1. When using a fixed-angle rotor, orient the device with the membrane panel facing up as demonstrated in the image below.

      For a more detailed view, kindly review the attached video link: https://youtu.be/GTH7kQMUjFg?feature=shared&t=145. This video also discusses the relative size of each Amicon® Ultra Centrifugal Filter and what kind of rotors they will fit into.

      Helpful?

  8. Hii, I wold like use these tubes for ultrafiltration of FBS to deplete it from EVs. I found they were used in one publication for such an experiment. How can they be sterilized so they can be used in cell culture hoods?

    1 answer

    1. Amicon Ultra devices cannot be sterilized by UV or autoclaving. These products are intended as a 1 time use device, however it is possible to sanitize the devices with 70% ethanol. Please see the protocol for sanitization below:

      1. Reagent preparation: 70% Ethanol solution (v/v) is prepared by dilution in distilled water. Both the ethanol solution and distilled water used for rinsing should be filter-sterilized using sterile, 0.22 µm sterilizing grade filter devices, such as the 50 mL Steriflip® device SCGP00525 or SE1M179M6.

      2.Sanitization Protocol:

      A. Fill the Amicon® Ultra concentrator device to its published capacity with filter-sterilized 70% Ethanol, and cap the unit (note that the collection tubes can be sanitized in the same manner).

      B. Invert device by hand or in an end-over-end mixer to continuously wet all internal surfaces of the device.

      C. Incubate for a minimum of 5 minutes (note that the device can be capped and left for up to 24 hours if it is not going to be used immediately).

      D. Spin at recommended G-force using appropriate centrifuge until at least 50% of the volume has passed through the device.

      E. Aseptically discard any remaining ethanol from the filter device and filter collection tube.

      F. Immediately rinse away residual ethanol by adding the filter-sterilized water to the device's published capacity, mix gently to wet all areas, and centrifuge until at least 50% of the volume has passed through the device.

      G. Discard any remaining liquid from both the concentrator device and filter collection tube before use.

      DO NOT let the filter membrane dry out in concentrator unit. If the devices are not to be used immediately, fill the devices with filter-sterilized distilled water, cover, and store refrigerated. Remove liquid from the device as previously described prior to use.

      Helpful?

  9. Hi, Can you please confirm the centrifugation speeds ( in rcf ) for the Amicon-15 100kDa and the 10kDa filters ? I was told that the max speed for the 100kDa is 3000g and for the 10kDa is 4000g, respectively. Is that the case and if so, why exactly ?

    1 answer

    1. Spin times range from 10 - 40 minutes and will depend on the sample types as well as the rotor (fixed-angle or swinging bucket). See the image below for spin times and speeds for each rotor type.
      Please see the link below to review the product FAQs for additional usage information:
      https://www.emdmillipore.com/US/en/product/Amicon-Ultra-15-Centrifugal-Filter-Unit,MM_NF-UFC910008#documentation

      To request the product user guide, we kindly ask you to navigate to the link https://www.sigmaaldrich.com/techservice, click on "Product Documentation" under the Products Section with all the required information so that a member of our team can reach out to you to assist further.

      Helpful?

  10. Please could you provide the manual for these kind of filters: Amicon Ultra 15 ml centrifugal filters

    1 answer

    1. Kindly review the user guide for these products at the following link: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/360/134/pr05035-rev1018.pdf

      Helpful?

1–10 of 12 Questions  

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