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MABE974

Sigma-Aldrich

Anti-Ser/Thr-protein kinase Nek2 Antibody, clone 5D2.1

clone 5D2.1, from mouse

Synonym(s):

Serine/threonine-protein kinase Nek2, Ser/Thr-protein kinase Nek2, HSPK 21, NimA-like protein kinase 1, Never in mitosis A-related kinase 2, NimA-related protein kinase 2

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

5D2.1, monoclonal

species reactivity

human, mouse

technique(s)

immunocytochemistry: suitable
western blot: suitable

isotype

IgG2bκ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... NEK2(4751)

General description

Serine/threonine-protein kinase Nek2 (EC 2.7.11.1; UniProt P51955; also known as HSPK 21, Never in mitosis A-related kinase 2, NimA-like protein kinase 1, NimA-related protein kinase 2, Protein phosphatase 1 regulatory subunit 111) is encoded by the NEK2 (also known as NEK2A, NLK1, PPP1R111, RP67) gene (Gene ID 4751) in human. Nek2 is a member of the never in mitosis A- (NIMA-) related family of serine/threonine kinases. Nek2 is involved in cell cycle regulation, gene expression, and the maintenance of centrosomal structure and function. Cellular Nek2 levels are tightly regulated throughout the cell cycle with noncentrosomal Nek2 expression peaks from S to G2 phase, while cytoplasmic Nek2 is highest in G1, and nuclear Nek2 is highest during S and G2 phase when it is needed for centrosomal function regulation. During interphase, centrosomes are held together by a proteinaceous linker that connects the the mother and daughter centriole. This linker is disassembled at the onset of mitosis in a process known as centrosome disjunction. The mammalian sterile 20-like kinase 2 (Mst2) and the scaffold protein Salvador (hSav1) are shown to directly interact with Nek2A and regulate its ability to localize to centrosomes, where Nek2A mediating linker dissociation by phosphorylating the centrosomal linker proteins C-Nap1 and rootletin. In addition, Nek2 is also reported to regulate gene expression by phosphorylating the splicing factor SRSF1. Nek2 expression is found upregulated in a wide variety of neoplasms including diffuse large B cell lymphoma and multiple myeloma.

Specificity

Expected to react with all four spliced isoforms of human Ser/Thr-protein kinase Nek2.

Immunogen

Epitope: N-terminal half
GST-tagged recombinant protein corresponding to the N-terminal of human Ser/Thr-protein kinase Nek2.

Application

Research Category
Epigenetics & Nuclear Function
Research Sub Category
Nuclear Receptors
This Anti-Ser/Thr-protein kinase Nek2 Antibody, clone 5D2.1 is validated for use in Western Blotting, Immunocytochemistry for the detection of Ser/Thr-protein kinase Nek2.
Western Blotting Analysis: 1.0 µg/mL from a representative lot detected Ser/Thr-protein kinase Nek2 in 10 µg of MOLT-4 and HL-60 cell lysates.
Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected Ser/Thr-protein kinase Nek2 in HeLa, NIH/3T3, and HUVEC cells.

Quality

Evaluated by Western Blotting in THP-1 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Ser/Thr-protein kinase Nek2 in 10 µg of THP-1 cell lysate.

Target description

~52/44 kDa observed (51.8 kDa/Nek2A (isoform 1), 44.9 kDa/Nek2B (isoform 2), 38.8 kDa/isoform 3, 50.9 kDa/Nek2C (isoform 4 or Nek2A-T), calculated). Uncharacterized band(s) may appear in some lysates.

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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