MABE1076
Anti-mono- ADP-ribose binding reagent
from Escherichia coli
Synonym(s):
Binding Reagent
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
Escherichia coli
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
monoclonal
species reactivity
human, mouse
packaging
antibody small pack of 25 μg
technique(s)
dot blot: suitable
western blot: suitable
shipped in
dry ice
target post-translational modification
unmodified
General description
Anti-mono-ADP-ribose binding reagent is a His-tagged recombinant protein fused to rabbit Fc tag, expressed in and purified from Rosetta (DE3)pLysS strain of E. coli (Cat. No. 70956). It is useful for the affinity detection of both mono-ADP-ribosylated proteins on membranes in a manner similar to antibody-based Western and dot blot analysis. The rabbit Fc tag allows visualization of the binding with conjugated anti-rabbit secondary antibodies. The Fc tag also allows Anti-ADP-ribose binding reagent to be captured on Protein A resins for affinity pull-down applications.
Specificity
This reagent binds to mono-ADP ribosylated proteins.
Application
Anti-mono-ADP-ribose binding reagent, Cat. No. MABE1076, is a reagent that targets mono (ADP-ribose) modified proteins and has been tested in Dot Blot and Western Blotting.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Western Blotting Analysis: 0.4 µg/mL of a representative lot detected mono-ADP-ribosylated proteins.
Dot Blot Analysis: A representative lot detected mono-,-ADP-ribose modified proteins.
Dot Blot Analysis: A representative lot detected mono-ADP ribosylated proteins. (Courtesy of Lee Kraus, University of Texas Southwestern Medical Center).
Western Blotting Analysis: A representative lot detected mono-ADP-ribose modified protein by Wester blotting (Courtesy of Lee Kraus, University of Texas Southwestern Medical Center).
Dot Blot Analysis: A representative lot detected mono-,-ADP-ribose modified proteins.
Dot Blot Analysis: A representative lot detected mono-ADP ribosylated proteins. (Courtesy of Lee Kraus, University of Texas Southwestern Medical Center).
Western Blotting Analysis: A representative lot detected mono-ADP-ribose modified protein by Wester blotting (Courtesy of Lee Kraus, University of Texas Southwestern Medical Center).
Quality
Evaluated by Gel Electrophoresis.
Gel Electrophoresis Analysis: 0.5 µg of this binding reagent was analyzed on GEL Electrophoresis to test for purity.
Gel Electrophoresis Analysis: 0.5 µg of this binding reagent was analyzed on GEL Electrophoresis to test for purity.
Target description
Variable depending on the target proteins and the extent of ADP-ribosylation.
Physical form
Format: Purified
Immobilized Metal Affinity Chromatography (IMAC)
Purified from E. coli and supplied in buffer containing 10 mM Tris pH 7.5, 0.2 M NaCl, 10 mM Imidazole, 1 mM PMSF, 1 mM beta-Mercaptoethanol, with 10% glycerol without preservatives.
Storage and Stability
Stable for 1 year at -80°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -80°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Lisa Weixler et al.
Life science alliance, 6(1) (2022-11-12)
The modification of substrates with ADP-ribose (ADPr) is important in, for example, antiviral immunity and cancer. Recently, several reagents were developed to detect ADP-ribosylation; however, it is unknown whether they recognise ADPr, specific amino acid-ADPr linkages, or ADPr with the
Yanmei Zhang et al.
Molecular cancer, 21(1), 158-158 (2022-08-03)
Brother of regulator of imprinted sites (BORIS) is expressed in most cancers and often associated with short survival and poor prognosis in patients. BORIS inhibits apoptosis and promotes proliferation of cancer cells. However, its mechanism of action has not been
Ning-Ning Zhang et al.
Oncology reports, 43(5), 1413-1428 (2020-04-24)
Colorectal cancer (CRC) is a global health concern. The role of epigenetics in tumors has garnered increasing interest. ADP ribosylation is an epigenetic modification that is associated with a variety of biological functions and diseases, and its association with tumor
Alvin Gomez et al.
The Biochemical journal, 475(23), 3827-3846 (2018-10-31)
Here, we report the biochemical characterization of the mono-ADP-ribosyltransferase 2,3,7,8-tetrachlorodibenzo-p-dioxin poly-ADP-ribose polymerase (TIPARP/ARTD14/PARP7), which is known to repress aryl hydrocarbon receptor (AHR)-dependent transcription. We found that the nuclear localization of TIPARP was dependent on a short N-terminal sequence and its
Travis Eisemann et al.
The Journal of biological chemistry, 294(40), 14574-14590 (2019-08-04)
Human tankyrase-1 (TNKS) is a member of the poly(ADP-ribose) polymerase (PARP) superfamily of proteins that posttranslationally modify themselves and target proteins with ADP-ribose (termed PARylation). The TNKS ankyrin repeat domain mediates interactions with a growing number of structurally and functionally
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