MAB4146
Anti-BCRP Antibody, clone BXP-21
culture supernatant, clone BXP-21, Chemicon®
Synonym(s):
Breast Cancer Resistance Protein, Placenta-specific ATP Binding Cassette Transporter, ATP-binding Cassette, Sub Family G, member 2 (ABCG2)
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
antibody form
culture supernatant
antibody product type
primary antibodies
clone
BXP-21, monoclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
input
sample type hematopoietic stem cell(s)
sample type neural stem cell(s)
isotype
IgG2a
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... ABCG2(9429)
General description
The 72 kDa breast cancer resistance protein (BCRP) is the second member of the subfamily G of the human ATP binding cassette (ABC) transporter superfamily and thus also designated as ABCG2. BCRP is a half-transporter consisting of only 1 nucleotide binding domain followed by 1 membrane-spanning domain. Current experimental evidence suggests that BCRP may function as a homodimer or homotetramer. Overexpression of BCRP is associated with high levels of resistance to a variety of anticancer agents. BCRP expression has been detected in a large number of hematological malignancies and solid tumors, indicating that this transporter may play an important role in clinical drug resistance of cancers. In addition to its role to confer resistance against chemotherapeutic agents, BCRP actively transports structurally diverse organic molecules, conjugated or unconjugated, such as estrone-3-sulfate, 17-β-estradiol 17-(β-D-glucuronide), and methotrexate. BCRP is highly expressed in the placental syncytiotrophoblasts, in the apical membrane of the epithelium in the small intestine, in the liver canalicular membrane, and at the luminal surface of the endothelial cells of human brain microvessels. This strategic and substantial tissue localization indicates that BCRP also plays an important role in absorption, distribution, and elimination of drugs that are BCRP substrates
Specificity
This antibody reacts with an internal epitope of the Breast Cancer Resistance Protein (BCRP), a 70 kDa transmembrane half-transporter which is involved in multidrug resistance. The antibody does not cross-react with human MDR1, MRP1, MRP2 or MRP5 gene products.
Immunogen
Fusion protein consisting of the E. coli maltose-binding protein and a 126-aa portion of BCRP (aa 271-396).
Application
Immunocytochemistry:
1:20 – 1:50 dilution of a previous lot was used on acetone fixed cytospin preparations.
Immunohistochemistry:
1:20 dilution of a previous lot was used on acetone fixed frozen tissue sections; 1:20 dilution of a previous lot was used on formaldehyde-fixed paraffin-embedded tissues pretreated with 10 mM citrate (pH 6.0) for 20 min.
Optimal working dilutions must be determined by end user.
1:20 – 1:50 dilution of a previous lot was used on acetone fixed cytospin preparations.
Immunohistochemistry:
1:20 dilution of a previous lot was used on acetone fixed frozen tissue sections; 1:20 dilution of a previous lot was used on formaldehyde-fixed paraffin-embedded tissues pretreated with 10 mM citrate (pH 6.0) for 20 min.
Optimal working dilutions must be determined by end user.
Research Category
Stem Cell Research
Stem Cell Research
Research Sub Category
Hematopoietic Stem Cells
Hematopoietic Stem Cells
This Anti-BCRP Antibody, clone BXP-21 is validated for use in IC, IH, IH(P), WB for the detection of BCRP.
Quality
Evaluated by Western Blot on HepG2 lysates.
Western Blot Analysis:
1:500 dilution of this antibody detected BCRP on 10 µg of HepG2 lysate.
Western Blot Analysis:
1:500 dilution of this antibody detected BCRP on 10 µg of HepG2 lysate.
Target description
70 kDa
Physical form
Mouse monoclonal IgG2a in buffer containing 0.7% BSA and 0.1% sodium azide
Unpurified
Storage and Stability
For long term storage, store at -20ºC. Stable for 6 months from date of receipt at 2-8ºC.
Analysis Note
Control
MCF7 cells
MCF7 cells
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Stefanie Hauswald et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 15(11), 3705-3715 (2009-05-22)
Histone deacetylase inhibitors (HDACi) are being studied in clinical trials with the aim to induce cellular differentiation, growth arrest, and apoptosis of tumor cells. Recent reports suggest that the multidrug resistance-1 (MDR1) gene is regulated by epigenetic mechanisms. To investigate
Hoang Dinh Tuy et al.
Oncology letters, 12(4), 2752-2760 (2016-10-05)
Irinotecan is a key drug for patients with advanced and recurrent colorectal carcinoma. However, the efficacy of irinotecan is not sufficient; partly, as there is no useful marker to predict chemosensitivity to the drug. The aim of the present study
Expression of BAF57 in ovarian cancer cells and drug sensitivity.
Yamaguchi, T; Kurita, T; Nishio, K; Tsukada, J; Hachisuga, T; Morimoto, Y; Iwai, Y; Izumi, H
Cancer Science null
Zhuo Chen et al.
Experimental eye research, 83(4), 798-806 (2006-06-24)
The development of vectors and techniques to transfer therapeutic genes to corneal epithelium has broad clinical applications. To determine if adenoviral (Ad5) vectors could be tailored to increase transduction of corneal epithelial progenitor cells expressing epidermal growth factor receptor (EGFR)
Phetcharawan Lye et al.
Journal of cellular and molecular medicine, 22(7), 3652-3660 (2018-04-25)
The placental multidrug transporters, P-glycoprotein (P-gp, encoded by ABCB1) and breast cancer resistance protein (BCRP, ABCG2) protect the foetus from exposure to maternally derived glucocorticoids, toxins and xenobiotics. During pregnancy, maternal glucocorticoid levels can be elevated by stress or exogenous
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