MAB1926-I

Anti-Fibronectin Antibody, cell binding domain Antibody, clone P1H11

clone P1H11, from mouse

• Synonym(s): FN, Cold-insoluble globulin, CIG
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: P1H11, monoclonal
• species reactivity: human, porcine, mouse
• packaging: antibody small pack of 25 μL
• technique(s): immunocytochemistry: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NM_002026
• UniProt accession no.: P02751
• shipped in: ambient
• target post-translational modification: unmodified
• Gene Information: human ... FN1(2335) ; mouse ... Fn1(14268)

Description

General description

Fibronectin (UniProt: P02751; also known as FN, Cold-insoluble globulin, CIG) is encoded by the FN1 (also known as FN) gene (Gene ID: 2335) in human. Fibronectin is are involved various biological activities, including cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. It is essential for osteoblast mineralization and participates in the regulation of type I collagen deposition by osteoblasts. Fibronectin is usually present as homodimer of ~250 kDa subunits where subunits are connected by two disulfide bonds at the C-terminal region. Each monomer contains three repeating units, which are designated as type I, II, and III repeats containing about 40, 60, and 90 amino acids, respectively. Plasma fibronectin (soluble dimeric form) is secreted by hepatocytes and the cellular fibronectin (dimeric or cross-linked multimeric forms) is produced by fibroblasts, epithelial and other cell types. Cellular fibronectin is deposited as fibrils in the extracellular matrix. Fibronectin is recognized by integrins alpha5beta1 and alphaVbeta3. The primary sequence motif of fibronectin for integrin binding is a tripeptide, Arg-Gly-Asp (RGD). Mutations in FN gene are known to cause glomerulopathy that is characterized by proteinuria, microscopic hematuria, and hypertension, which can lead to end-stage renal failure.

Specificity

Clone P1H11 detects fibronectin in human.

Immunogen

Human Plasma Fibronectin.

Application

Anti-Fibronectin, cell binding domain, clone P1H11, Cat. No. MAB1926-I is a mouse monoclonal antibody that detects human fibronectin and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Fibronectin in human kidney and human cerebellum tissues.

Western Blotting Analysis: A representative lot detected Fibronectin in Western Blotting applications (Zhang, H., et. al. (2010). Am J Physiol Renal Physiol. 299(1):F91-8).

Immunocytochemistry Analysis: A representative lot detected Fibronectin in Immunocytochemistry applications (Mardilovich, A., et. al. (2006). Langmuir. 22(7):3259-64).

Immunocytochemistry Analysis: A representative lot detected Fibronectin in Immunocytochemistry applications (Hofanson, D.M., et. al. (2010). Biomaterials. 31(26):6730-7).

Immunocytochemistry Analysis: A representative lot detected Fibronectin in Immunocytochemistry applications (Waterman, R.S., et. al. (2010). PLoS One. 5(4):e10088).

Immunohistochemistry Analysis: A representative lot detected Fibronectin in Immunohistochemistry applications (Carlos, C.P., et. al. (2014). PLoS One. 9(7):e103660).

Western Blotting Analysis: A representative lot detected Fibronectin in Western Blotting applications (Zhang, H., et. al. (2008). Am J Physiol Renal Physiol. 295(4):F1071-81).

Research Category
Cell Structure

Quality

Evaluated by Western Blotting in human thymus tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Fibronectin in 10 µg of human thymus tissue lysate.

Target description

~270 kDa observed; 262.62 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable