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MAB1922Z

Sigma-Aldrich

Anti-Laminin α2 Antibody, clone 5H2, Azide Free

clone 5H2, Chemicon®, from mouse

Synonym(s):

MerosinMchain, MAB1922

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

5H2, monoclonal

species reactivity

human, rabbit, monkey

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

NM_000426.3
NM_001079823.1

UniProt accession no.

P24043

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... LAMA2(3908)
rhesus monkey ... Lama2(715394)

Specificity

Reacts with the 80 kDa fragment of the M-chain of human merosin.

SPECIES REACTIVITIES:

Cross reacts with monkey and rabbit merosin.

Immunogen

Purified human merosin.

Application

Anti-Laminin α2 Antibody, clone 5H2, Azide Free detects level of Laminin α2 & has been published & validated for use in ELISA, IH, IP & WB.
ELISA: 50% maximal binding to human merosin at 1:50,000.

Immunohistochemistry: 1:5,000 for staining of 8 mm acetone-fixed cryostat muscle sections, prior to detection with a peroxidase-conjugated secondary antibody.

Immunofluorescence

Affinity chromatography

Immunoprecipitation

Immunoblotting

Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Research Sub Category
ECM Proteins

Physical form

Format: Purified

Storage and Stability

Maintain between 2 and 8°C for up to 6 months.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Corinna Preuße et al.
Acta neuropathologica communications, 4(1), 45-45 (2016-04-29)
Dermatomyositis (DM) can occur in both adults and juveniles with considerable clinical differences. The links between immune-mediated mechanisms and vasculopathy with respect to development of perifascicular pathology are incompletely understood. We investigated skeletal muscle from newly diagnosed, treatment-naïve juvenile (jDM)
Elizabeth I Tang et al.
Endocrinology, 163(11) (2022-08-17)
Microtubule affinity-regulating kinases (MARKs) are nonreceptor Ser/Thr protein kinases known to regulate cell polarity and microtubule dynamics in Caenorhabditis elegans, Drosophila, invertebrates, vertebrates, and mammals. An earlier study has shown that MARK4 is present at the ectoplasmic specialization and blood-testis

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