Reacts with HBcAg (epitope recognized - amino acid positions 2-8).
Immunogen
Epitope: Core Antigen (ayw)
Recombinant purified HBcAg from E.coli, SDS-denatured
Application
Anti-Hepatitis B Virus Antibody, core antigen (ayw), clone 10E11, a.a. 2-10 detects level of Hepatitis B Virus & has been published & validated for use in ELISA, IF, IP, WB, IC, IH.
ELISA, immunoblotting, immunofluorescent and immunoperoxidase staining of infected liver tissues and transfected cultured cells. Optimal working dilutions: for immunoblotting - 1:1000; for immunofluorescence - 1:200. Optimal working dilutions must be determined by the end user.
Research Category Infectious Diseases
Research Sub Category Infectious Diseases - Viral
Physical form
Ascitic fluid. Liquid in DMEM, containing 0.1% sodium azide.
Storage and Stability
Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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An E. coli expression system offers a mean for rapid, high yield and economical production of Hepatitis B Virus core (HBc) particles. However, high-level production of HBc particles in bacteria is demanding and optimisation of HBc particle yield from E.
Differentiation of core gene products of the hepatitis B virus in infected liver tissue using monoclonal antibodies
Naoumov, N V, et al
Journal of Medical Virology, 53, 127-138 (1997)
The affinities of monoclonal antibodies against core antigen of hepatitis B virus.
Particulate and denatured core protein as well as e-antigen (HBe) of hepatitis B virus (HBV) differ in part immunologically but this has not been studied in sufficient detail. Therefore, in this study the B-cell immune response to native and denatured
Inflammation research : official journal of the European Histamine Research Society ... [et al.], 69(7), 683-696 (2020-04-30)
Hepatitis B virus X protein (HBx) is a pivotal factor for HBV-induced hepatitis. Herein, we sought to investigate HBx-mediated NLR pyrin domain containing 3 (NLRP3) inflammasome activation and pyroptosis under oxidative stress. The effect of HBx on the NLRP3 inflammasome
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