ABN1464

Anti-GRP-78

from rabbit, purified by affinity chromatography

• Synonym(s): 78 kDa glucose-regulated protein, GRP-78, Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78, Heat shock 70 kDa protein 5, Immunoglobulin heavy chain-binding protein, BiP
• UNSPSC Code: 12352203
• eCl@ss: 32160702

Properties

• biological source: rabbit
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human
• species reactivity (predicted by homology): ground squirrel (based on 100% sequence homology), hamster (based on 100% sequence homology), bovine (based on 100% sequence homology), mouse (based on 100% sequence homology), goat (based on 100% sequence homology), primate (based on 100% sequence homology)
• technique(s): immunocytochemistry: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• NCBI accession no.: NP_005338
• UniProt accession no.: P11021
• shipped in: ambient
• target post-translational modification: unmodified
• Gene Information: human ... HSPA5(3309)

Description

General description

78 kDa glucose-regulated protein (UniProt: P11021; also known as GRP-78, Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78, Heat shock 70 kDa protein 5, Immunoglobulin heavy chain-binding protein, BiP) is encoded by the HSPA5 (also known as GRP78) gene (Gene ID: 3309) in human. GRP78 a member of the HSP family of molecular chaperones that is required for stress-induced autophagy and for endoplasmic reticulum (ER) integrity. GRP78 is involved in multiple processes, including translocation of the newly formed proteins across the ER membrane, folding and assembly of proteins, and targeting misfolded proteins for ER-associated degradation. It plays a critical role in regulating unfolded protein response (UPR). Following ER stress, GRP78 is released from ER transmembrane signal transducers, such as PKR-like ER kinase (PERK), which leads to the activation of UPR signaling pathways. Once GRP78 is dissociated, PERK is allowed to dimerize and is autophosphorylated and activated. Activated PERK phosphorylates elF2alpha that results in the reduction of protein synthesis. (Ref.:Wang, M., et al. (2009). Antioxid Redox Signal. 11(9): 2307 2316).

Specificity

This polyclonal antibody detects GRP-78 protein in multiple mammalian species. It targets an epitope within 17 amino acids from the C-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 17 amino acids from the C-terminal region of human GRP-78.

Application

Anti-GRP-78, Cat. No. ABN1464, is a highly specific rabbit polyclonal antibody that targets 78 kDa glucose-regulated protein and has been tested in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Research Category
Neuroscience

Western Blotting Analysis: 0.2 µg/mL from a representative lot detected GRP-78 in 10 µg of A431 cell lysates.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected GRP-78 in human cerebellum, human pancreas, human small intestine, and human tonsil tissues.

Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected GRP-78 in NIH/3T3 cells.

Quality

Evaluated by Western Blotting in A431 cell lysates.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected GRP-78 in 10 µg of A431 cell lysates.

Target description

~78 kDa observed; 72.33 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Affinity Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable