AB3203
Anti-Tubulin Antibody, δ 2
Chemicon®, from rabbit
Synonym(s):
Anti-α-tubulin, Tubulin antibody
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
sea urchin, echinoderm, plant
should not react with
Saccharomyces cerevisiae
species reactivity (predicted by homology)
mammals
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... TUBD1(51174)
General description
Tubulin, the dimeric subunit of microtubules, is submitted to post translational modifications including cyclic tyrosine removal and addition at the COOH terminus of the alpha subunit. The cycle involves two enzymes, TTL (Tubuline Tyrosine Ligase) and Tubulin carboxypeptidase, and generates two major forms: tyrosinated tubulin and detyrosinated tubulin (Glu-tubulin). Removal of the COOH-terminal Glu residue from the alpha chain of Glu-tubulin generates the delta2-tubulin form.
Specificity
The antibody specifically recognizes the delta 2 form of the tubulin alpha chain (Paturle-Lafanechere et al. 1991).
SPECIES REACTIVITY:
Mammals, sea urchin, plants, not yeast (Lafanechere et al. 1994).
SPECIES REACTIVITY:
Mammals, sea urchin, plants, not yeast (Lafanechere et al. 1994).
Immunogen
Peptide corresponding to the C-terminal of delta 2 tubulin.
Application
Research Category
Cell Structure
Cell Structure
Research Sub Category
Cytoskeleton
Cytoskeleton
This Anti-Tubulin Antibody, δ 2 is validated for use in IH(P), WB, IC, IH for the detection of Tubulin.
Western blot: 1:1,000
Immunohistochemistry and EM on either frozen or paraffin embedded, fixed tissue (Mialhe et al. 2001): 1:500
Optimal working dilutions must be determined by the end user.
Immunohistochemistry and EM on either frozen or paraffin embedded, fixed tissue (Mialhe et al. 2001): 1:500
Optimal working dilutions must be determined by the end user.
Physical form
Affinity purified immunoglobulin. Liquid in PBS.
Storage and Stability
Maintain at 2-8°C in undiluted for up to 6 months from date of receipt.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Robert O'Hagan et al.
Current biology : CB, 21(20), 1685-1694 (2011-10-11)
Posttranslational modifications (PTMs) such as acetylation, detyrosination, and polyglutamylation have long been considered markers of stable microtubules and have recently been proposed to guide molecular motors to specific subcellular destinations. Microtubules can be deglutamylated by the cytosolic carboxypeptidase CCP1. Loss
Kinesin-13 and tubulin posttranslational modifications regulate microtubule growth in axon regeneration.
Ghosh-Roy, A; Goncharov, A; Jin, Y; Chisholm, AD
Developmental Cell null
Post-translational modifications to Toxoplasma gondii alpha- and beta-tubulins include novel C-terminal methylation.
Xiao, H; El Bissati, K; Verdier-Pinard, P; Burd, B; Zhang, H; Kim, K; Fiser, A; Angeletti et al.
Journal of Proteome Research null
Hung-Chi Liu et al.
Plant physiology, 191(1), 177-198 (2022-10-23)
Phosphoglucose isomerase (PGI) catalyzes the interconversion of fructose-6-phosphate and glucose-6-phosphate, which impacts cell carbon metabolic flow. Arabidopsis (Arabidopsis thaliana) contains two nuclear PGI genes respectively encoding plastidial PGI1 and cytosolic PGI (cPGI). The loss of PGI1 impairs the conversion of
Dynamics of DNA methylation and Histone H4 acetylation during floral bud differentiation in azalea.
Meijon, M; Feito, I; Valledor, L; Rodriguez, R; Ca?al, MJ
BMC plant biology null
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