5.04906

Cuspin-1

• Synonym(s): Cuspin-1, (5-Bromo-3-pyridinyl)(4-methylphenyl)methanone, Survival Motor Neuron protein Upregulator, Cuspin-1
• Empirical Formula (Hill Notation): C₁₃H₁₀BrNO
• CAS Number: 337932-29-3
• Molecular Weight: 276.13
• UNSPSC Code: 12352200

Properties

• Assay: ≥94% (HPLC)
• Quality Level: 100
• form: powder
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; protect from light
• color: brown
• solubility: DMSO: 10 mg/mL
• storage temp.: 2-8°C

Description

General description

A cell-permeable pyridinylphenyl-methanone compound that is reported to effectively upregulate SMN (survival of motor neuron) protein level in 3 out of 4 SMA (spinal muscular atrophy) patient-derived fibroblast lines (by 71% to 93% increase in 48 h; 5 µg/mL), but not HT1080, 293T, A549, two human ESC-MN lines, MEF, mESC-MN, rat PC12, or rat ST14A cells. Shown to preferentially stimulate cellular Erk (Thr202/Tyr204) over Akt (Ser473) phosphorylation in SMA fibroblast line 9677 in a time-dependent manner (t_max = 24 h; 10 µg/mL), indicative of Ras-Raf-MEK signaling pathway activation.

Please note that the molecular weight for this compound is batch-specific due to variable water content.

Biochem/physiol Actions

Cell permeable: yes

Primary Target
Ras

Reversible: yes

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Other Notes

Letso, R.R., et al. 2013. ACS Chem. Biol.8, 914.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3