454850-M

MAP Kinase 2, Mouse, Recombinant, E. coli

• Synonym(s): ERK2, MAPK2, p42 ^MAPK
• UNSPSC Code: 12352202

Properties

• recombinant: expressed in E. coli
• Quality Level: 100
• Assay: ≥90% (SDS-PAGE)
• form: liquid
• specific activity: ≥5000 units/mL
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• foreign activity: MEK, phosphatase, protease, none detected
• storage temp.: −70°C

Description

General description

Recombinant, mouse MAP Kinase 2 expressed in E. coli. A serine/threonine protein kinase that acts at the end of a protein cascade linking signals from cell surface receptor tyrosine kinases to cytoplasmic and nuclear events. This protein was co-expressed with MEK and is phosphorylated and active.

Recombinant, mouse MAP Kinase 2 expressed in E. coli. A serine/threonine protein kinase that acts at the end of a protein kinase cascade linking signals from cell surface receptor tyrosine kinases to cytoplasmic and nuclear events. This protein was co-expressed with MEK and is phosphorylated and active.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will catalyze the transfer of 1.0 pmol phosphate to myelin basic protein per min at 30°, pH 7.5.

Physical form

In 100 mM NaCl, 50 mM HEPES, 1 mM DTT, 100 µM Na₂-EDTA, 50% glycerol, 0.01% BRIJ^® 35 detergent, pH 7.5.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Fukunaga, K., and Miyamoto, E. 1998. Mol. Neurobiol. 16, 79.
Wu, J., et al. 1993. Mol. Cell. Biol.13, 4539.

Legal Information

Brij is a registered trademark of Croda International PLC

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable